The Effects of UV Emission from Compact Fluorescent Light Exposure on Human Dermal Fibroblasts and Keratinocytes In Vitro
The Effects of UV Emission from Compact Fluorescent Light Exposure on Human Dermal Fibroblasts and Keratinocytes In Vitro
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DOI：
10.1111/j.1751-1097.2012.01192.x
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发表时间：
2012-11-01
影响因子：
3.3
通讯作者：
Rafailovich, Miriam H.
中科院分区：
文献类型：
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作者：
Mironava, Tatsiana;Hadjiargyrou, Michael;Rafailovich, Miriam H.
紧凑型荧光灯（CFL）灯泡可以提供与白炽灯泡相同的流明，使用四分之一的能量。最近，CFL暴露被发现会加剧现有的皮肤状况;然而，CFL暴露对健康皮肤组织的影响尚未得到彻底研究。在这项研究中，我们研究了暴露于CFL照明对健康人体皮肤组织细胞（成纤维细胞和角质形成细胞）的影响。暴露于CFL的细胞表现出增殖率下降，活性氧的产生显着增加，并在其收缩胶原蛋白的能力下降。对这些灯泡的紫外线辐射进行测量发现，所有研究的灯泡中都存在明显的UVC和UVA（汞[Hg]发射线），这似乎源于荧光粉涂层的裂缝。细胞对CFL的反应与紫外线辐射的损伤一致，当暴露前加入低剂量的通常用于紫外线吸收的TiO 2纳米颗粒（NPs）时，这种损伤进一步增强。当细胞暴露于相同强度的白炽光时，没有观察到对具有或不具有TiO 2 NPs的细胞的影响。
Compact fluorescent light (CFL) bulbs can provide the same amount of lumens as incandescent light bulbs, using one quarter of the energy. Recently, CFL exposure was found to exacerbate existing skin conditions; however, the effects of CFL exposure on healthy skin tissue have not been thoroughly investigated. In this study, we studied the effects of exposure to CFL illumination on healthy human skin tissue cells (fibroblasts and keratinocytes). Cells exposed to CFLs exhibited a decrease in the proliferation rate, a significant increase in the production of reactive oxygen species, and a decrease in their ability to contract collagen. Measurements of UV emissions from these bulbs found significant levels of UVC and UVA (mercury [Hg] emission lines), which appeared to originate from cracks in the phosphor coatings, present in all bulbs studied. The response of the cells to the CFLs was consistent with damage from UV radiation, which was further enhanced when low dosages of TiO2 nanoparticles (NPs), normally used for UV absorption, were added prior to exposure. No effect on cells, with or without TiO2 NPs, was observed when they were exposed to incandescent light of the same intensity.