Adrenomedullin in adipocyte differentiation of human mesenchymal stem cells.
Adrenomedullin in adipocyte differentiation of human mesenchymal stem cells.
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DOI：
10.1016/j.bbrc.2006.09.089
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发表时间：
2006-11
影响因子：
3.1
通讯作者：
Yin Li;Yan Zhang;S. Shibahara;Kazuhiro Takahashi
中科院分区：
文献类型：
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作者：
Yin Li;Yan Zhang;S. Shibahara;Kazuhiro Takahashi
研究了人间充质干细胞（hMSCs）脂肪细胞分化过程中强效血管舒张肽肾上腺髓质素（AM）的表达。在脂肪细胞分化的第4天和第8天，培养基中的免疫反应性AM水平升高。Northern blot分析显示，在第4、8、12和18天，hmscs来源的脂肪细胞中AM mRNA的表达增加。瞬时转染实验表明，用含有人AM基因顺式作用区（- 70/ - 29）的质粒转染hMSCs来源的脂肪细胞时，启动子活性高于hMSCs。电泳迁移率转移实验表明，在hMSCs来源的脂肪细胞中，特定的条带结合到区域（- 70/ - 29），而在hMSCs中没有，并且被刺激蛋白1 （Sp1）抗体消除。本研究表明，在hMSCs的脂肪细胞分化过程中，AM的表达上调可能是通过Sp1或Sp1相关因子(s)与AM启动子区（−70/−29）的相互作用。
Expression of adrenomedullin (AM), a potent vasodilator peptide, was studied during adipocyte differentiation of human mesenchymal stem cells (hMSCs). Immunoreactive AM levels in the medium were increased at day 4 and 8 of the adipocyte differentiation. Northern blot analysis showed increased expression of AM mRNA in hMSCs-derived adipocytes at day 4, 8, 12, and 18. Transient transfection assay showed that the promoter activity was higher in hMSCs-derived adipocytes than in hMSCs, when cells were transfected with plasmids containing a cis-acting region (−70/−29) of the human AM gene. Electrophoretic mobility shift assay showed that specific bands bound to the region (−70/−29) in hMSCs-derived adipocytes but not in hMSCs, and were abolished by the stimulatory protein 1 (Sp1) antibody. The present study has shown that AM expression is up-regulated during adipocyte differentiation of hMSCs probably via the interaction between Sp1 or Sp1-related factor(s) and the AM promoter region (−70/−29).