Protein-protein interaction in purple membrane.
Protein-protein interaction in purple membrane.
复制标题
紫色膜中蛋白质-蛋白质相互作用。
DOI：
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发表时间：
2009
影响因子：
8.6
通讯作者：
D. Strauch
中科院分区：
文献类型：
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作者：
M. Rheinstädter;K. Schmalzl;K. Wood;D. Strauch
我们提出了紫膜（PM）中的长程蛋白质-蛋白质相互作用的实验证据。利用非弹性中子散射测量二维细菌视紫红质（BR）蛋白质晶格中的声学声子谱，定量了蛋白质间的动力学。声子能量约为1毫电子伏的测定。将数据与分析模型进行比较，并确定相邻蛋白质三聚体之间相互作用的有效弹簧常数为k = 53 N/m。此外，光学样激发在0.45毫电子伏被发现，并分配到相邻的BR单体之间的蛋白质内动力学。
We present experimental evidence for a long-range protein-protein interaction in purple membrane (PM). The interprotein dynamics were quantified by measuring the spectrum of the acoustic phonons in the 2D bacteriorhodopsin (BR) protein lattice using inelastic neutron scattering. Phonon energies of about 1 meV were determined. The data are compared to an analytical model, and the effective spring constant for the interaction between neighboring protein trimers are determined to be k = 53 N/m. Additional, optical-like excitations at 0.45 meV were found and assigned to intraprotein dynamics between neighboring BR monomers.