Microfluidic-based cell sorting of Francisella tularensis infected macrophages using optical forces
Microfluidic-based cell sorting of Francisella tularensis infected macrophages using optical forces
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DOI：
10.1021/ac8007779
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发表时间：
2008-08-15
影响因子：
7.4
通讯作者：
Patel, Kamiesh D.
中科院分区：
文献类型：
--
作者：
Perroud, Thomas D.;Kaiser, Julia N.;Patel, Kamiesh D.
我们已经扩展了光镊的原理，作为一种非侵入性的技术，在微流体装置中基于它们的荧光信号来主动地分选流体动力学聚焦的细胞。这种微荧光激活细胞分选仪（mu FACS）使用红外激光将细胞横向偏转到收集通道中。绿色标记的巨噬细胞从40/60比例的混合物中以22个细胞/s的通量分选30分钟，实现93%的分选纯度和60%的回收率。为了排除光偏转过程中潜在的光诱导细胞损伤，我们研究了小鼠巨噬细胞对短暂暴露的反应（
We have extended the principle of optical tweezers as a noninvasive technique to actively sort hydrodynamically focused cells based on their fluorescence signal in a microfluidic device. This micro fluorescence-activated cell sorter (mu FACS) uses an infrared laser to laterally deflect cells into a collection channel. Green-labeled macrophages were sorted from a 40/60 ratio mixture at a throughput of 22 cells/s over 30 min achieving a 93% sorting purity and a 60% recovery yield. To rule out potential photoinduced cell damage during optical deflection, we investigated the response of mouse macrophage to brief exposures (