Effects of microtubule-associated protein (MAP) expression on methylmercury-induced microtubule disassembly.
Effects of microtubule-associated protein (MAP) expression on methylmercury-induced microtubule disassembly.
复制标题
微管相关蛋白（MAP）表达对甲基汞诱导的微管解体的影响。
DOI：
10.1006/taap.2000.8953
复制
发表时间：
2000
影响因子：
3.8
通讯作者：
D. Brown
中科院分区：
文献类型：
--
作者：
A. M. Hunter;D. Brown
比较了未分化、MAP1A和MAP2C转染和神经元分化的P19胚胎癌（EC）细胞中微管（MT）对甲基汞（MeHg）诱导的解体的敏感性。通过免疫荧光显微镜和Western印迹定性检测MT分解的程度，并通过聚合物和可溶性蛋白提取物的斑点印迹定量检测MT分解的程度。免疫荧光显微镜显示，甲基汞拆解MT在时间和剂量依赖性的方式和MT在MAP 2C转染和神经元分化的细胞，但不是那些在MAP 1A转染的细胞，显着更耐甲基汞诱导的MT解聚比那些在未分化的细胞。这些结果表明，MAP2C比MAP1A具有更大的稳定MT对抗甲基汞诱导的解体的能力。然而，令人惊讶的是，当通过Western印迹和定量斑点印迹评估MT分解的程度时，与未处理的对照细胞相比，在甲基汞处理的样品中的聚合物和可溶性级分中，没有观察到微管蛋白、MAP 2或MAP 1A的量的变化。这些数据表明，虽然甲基汞处理导致的拆卸的MT，他们没有解聚为洗涤剂可溶性亚基，而是出现形成不溶性微管蛋白MAP低聚物或聚集体。
The sensitivity of microtubules (MTs) to methylmercury- (MeHg) induced disassembly was compared in undifferentiated, MAP1A- and MAP2C-transfected, and neuronally differentiated P19 Embyronal Carcinoma (EC) cells. The extent of MT disassembly was examined qualitatively by immunofluorescence microscopy and Western blotting and quantitatively by dot blotting of polymer and soluble proteins extracts. Immunofluorescence microscopy showed that MeHg disassembled MTs in a time- and dose-dependent manner and that MTs in both MAP2C-transfected and neuronally differentiated cells, but not those in MAP1A-transfected cells, were significantly more resistant to MeHg-induced MT depolymerization than those in undifferentiated cells. These results suggest that MAP2C has a greater ability to stabilize MTs against MeHg-induced disassembly than MAP1A. Surprisingly, however, when the extent of MT disassembly was assessed by Western blotting and by quantitative dot blotting, no change was observed in the amounts of tubulin, MAP2, or MAP1A, in the polymer and soluble fractions in MeHg-treated samples, compared to the control cells that were not treated. These data show that, although MeHg treatment resulted in the disassembly of MTs, they were not depolymerized as detergent-soluble subunits, but rather appeared to form insoluble tubulin-MAP oligomers or aggregates.
神经元微管稳定性的分子基础。
影响因子：
3.4
作者：
Falconer,MM;Vaillant,A;Reuhl,KR;Laferriere,N;Brown,DL
通讯作者：
Brown,DL
影响因子：
3.8
作者：
Vogel,DG;Margolis,RL;Mottet,NK
通讯作者：
Mottet,NK
影响因子：
3.8
作者：
Graff, RD;Falconer, MM;Reuhl, KR
通讯作者：
Reuhl, KR