Mapping and identification of candidate loci responsible for Peromyscus hybrid overgrowth
Mapping and identification of candidate loci responsible for Peromyscus hybrid overgrowth
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DOI：
10.1007/s00335-006-0083-x
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发表时间：
2007-01-01
期刊：
影响因子：
2.5
通讯作者：
Vrana, Paul B.
中科院分区：
文献类型：
--
作者：
Loschiavo, Maria;Nguyen, Quang K.;Vrana, Paul B.
两个最近分化的啮齿类动物物种的属Peromyscus之间的杂交结果在戏剧性的父母的原产地的影响，对生长和发育。P. maniculatus雌性与P. polionotus雄性杂交产生生长迟缓的孕体，而相反的杂交导致过度生长和致死。这些混合效应在胎盘中特别明显。我们先前检测到与过度生长效应相关的基因组两个区域的连锁。一个基因座，称为Peal，是一个父系表达的常染色体基因座映射到一个域，其家鼠相当于包含几个集群的印记基因。另一个基因座，称为Mexl，定位于X染色体的基因贫乏区域。在这里，我们使用一个先进的互交系，以验证和缩小区域的连锁，并确定候选基因Mexl和Peal。虽然我们以前已经表明，Mexl影响产前和产后的增长，我们在这里显示，Peal只影响产前的增长。利用标准，如突变表型和等位基因表达，我们确定的基因座编码的同源框蛋白Esx 1和锌指蛋白Pw 1/Peg 3作为候选人。这两个基因座在杂种中表现出表达变化。
Crosses between two recently diverged rodent species of the genus Peromyscus result in dramatic parent-of-origin effects on growth and development. P. maniculatus females crossed with P. polionotus males yield growth-retarded conceptuses, whereas the reciprocal cross results in overgrowth and lethality. These hybrid effects are particularly pronounced in the placenta. We previously detected linkage to two regions of the genome involved in the overgrowth effects. One locus, termed Peal, is a paternally expressed autosomal locus mapping to a domain whose house mouse equivalent contains several clusters of imprinted genes. The other locus, termed Mexl, maps to a gene-poor region of the X chromosome. Here we use an advanced intercross line to verify and narrow the regions of linkage and identify candidate genes for Mexl and Peal. While we have previously shown that Mexl affects both pre-and postnatal growth, we show here that Peal affects only prenatal growth. Utilizing criteria such as mutant phenotypes and allelic expression, we identify the loci encoding the homeobox protein Esx1 and the zinc-finger protein Pw1/Peg3 as candidates. Both loci exhibit expression changes in the hybrids.