Analysis of alkaloids in Coptis chinensis Franch by accelerated solvent extraction combined with ultra performance liquid chromatographic analysis with photodiode array and tandem mass spectrometry detections
Analysis of alkaloids in Coptis chinensis Franch by accelerated solvent extraction combined with ultra performance liquid chromatographic analysis with photodiode array and tandem mass spectrometry detections
复制标题
加速溶剂萃取结合光电二极管阵列超高效液相色谱分析和串联质谱检测分析黄连中的生物碱
DOI：
10.1016/j.aca.2008.02.060
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发表时间：
2008-04-21
影响因子：
6.2
通讯作者：
Yang, Huanghao
中科院分区：
文献类型：
--
作者：
Chen, Junhui;Wang, Fenymei;Yang, Huanghao
开发了一种基于加速溶剂萃取 (ASE) 和超高效液相色谱 (UPLC) 分析的新方法，用于鉴定和定量黄连提取物中的主要生物碱。 UPLC系统由光电二极管阵列检测器(PDA)和正离子电喷雾电离-串联质谱(ESI-MS/MS)的双检测系统按顺序配置组成。对提取溶剂、提取温度、静态提取时间和提取周期等ASE操作参数进行了优化。 UPLC 分析在 ACQUITY UPLC BEH C-18 柱上进行，洗脱液为乙腈流动相，其中加入了由 0.50% 乙酸和 20 mmol L-1 乙酸铵组成的缓冲溶液。采用全扫描模式或 MS/MS 模式进行多反应监测 (MRM) 的串联四极杆光谱仪用于对中华茶提取物中的八种主要生物碱进行鉴定和定量分析。样品还通过高效液相色谱-电喷雾电离-飞行时间质谱（HPLC-ESI-TOF-MS）系统进行分析，以确认鉴定结果。通过 UPLC-PDA 和 UPLC-MS/MS 对八种主要生物碱中的三种：小檗碱、巴马汀和药根碱进行定量。结果表明，UPLC-PDA和UPLC-MS/MS方法简便、灵敏、可靠，适用于羊草中生物碱的含量测定。使用既定方法对来自不同地点的七个黄连样品进行了分析。基于八种主要生物碱分布的UPLC指纹图谱可以为中药材的鉴定和质量评价提供快速可靠的方法。 (C) 2008 Elsevier B.V. 保留所有权利。
A new method based on accelerated solvent extraction (ASE) followed by ultra performance liquid chromatography (UPLC) analysis has been developed for the identification and quantification of major alkaloids in extracts of Coptis chinensis Franch. The UPLC system consisted of a dual detection system of photodiode array detector (PDA) and positive ion electrospray ionization-tandem mass spectrometry (ESI-MS/MS) in sequential configuration. The operational parameters of ASE including extraction solvent, extraction temperature, static extraction time and extraction cycles were optimized. UPLC analysis was performed on an ACQUITY UPLC BEH C-18 column eluted by a mobile phase of acetonitrile spiked with a buffer solution consisting of 0.50% acetic acid and 20 mmol L-1 ammonium acetate. A tandem quadrupole spectrometer operating in either full scan mode or in MS/MS mode for multiple reaction monitoring (MRM) was used for the identification and quantitative analysis of eight major alkaloids in C. chinensis Franch extracts. The samples were also analyzed on a high-performance liquid chromatography-electrospray ionization-time-of-flight mass spectrometry (HPLC-ESI-TOF-MS) system to confirm the identification results. Three of the eight major alkaloids, berberine, palmatine and jatrorrhizine were quantified by UPLC-PDA and UPLC-MS/MS. The results indicated that both UPLC-PDA and UPLC-MS/MS methods were simple, sensitive and reliable for the determination of alkaloids in C. chinensis Franch. Seven Huanglian samples from different locations were analyzed using the established methods. UPLC fingerprints based on the distribution of the eight major alkaloids can serve as a rapid and reliable method for the authentication and quality evaluation of traditional Chinese medicine (TCM) herbs. (C) 2008 Elsevier B.V. All rights reserved.