Hox10-regulated endodermal cell migration is essential for development of the ascidian intestine
Hox10-regulated endodermal cell migration is essential for development of the ascidian intestine
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DOI：
10.1016/j.ydbio.2015.03.018
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发表时间：
2015-07-01
影响因子：
2.7
通讯作者：
Sasakura, Yasunori
中科院分区：
文献类型：
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作者：
Kawai, Narudo;Ogura, Yosuke;Sasakura, Yasunori
Hox簇基因在后生动物前后轴的发育中起着至关重要的作用。Hox基因在中枢神经系统和肢体芽定形中的功能是众所周知的。它们也在脊索动物的内胚层组织中表达，但它们在内胚层发育中的作用仍然知之甚少。无脊椎脊索动物（Ciona ninteinalis）的内胚层组织在幼虫期处于早熟状态，在变态期分化为消化道。在这项研究中，我们发现Hox基因Ci-Hox10的破坏可以阻止肠道的形成。ci - hox10敲除的幼虫表现出内胚层链细胞迁移缺陷。对细胞迁移很重要的突起的形成在这些细胞中被破坏。胶原IX型基因是Ci-Hox10的下游靶点，在内胚层细胞迁移之前，受到Ci-Hox10和基质金属蛋白酶同源物的负调控。抑制这种调节可以阻止细胞迁移。这些结果表明，Ci-Hox10通过形成突起和重建细胞外基质来调节内胚层链细胞的迁移。(C) 2015爱思唯尔公司版权所有。
Hox cluster genes play crucial roles in development of the metazoan antero-posterior axis. Functions of Hox genes in patterning the central nervous system and limb buds are well known. They are also expressed in chordate endodermal tissues, where their roles in endodermal development are still poorly understood. In the invertebrate chordate, Ciona intestinalis, endodermal tissues are in a premature state during the larval stage, and they differentiate into the digestive tract during metamorphosis. In this study, we showed that disruption of a Hox gene, Ci-Hox10, prevented intestinal formation. Ci-Hox10-knock-down larvae displayed defective migration of endodermal strand cells. Formation of a protrusion, which is important for cell migration, was disrupted in these cells. The collagen type IX gene is a downstream target of Ci-Hox10, and is negatively regulated by Ci-Hox10 and a matrix metalloproteinase ortholog, prior to endodermal cell migration. Inhibition of this regulation prevented cellular migration. These results suggest that Ci-Hox10 regulates endodermal strand cell migration by forming a protrusion and by reconstructing the extracellular matrix. (C) 2015 Elsevier Inc. All rights reserved.