Synthesis of adenosine functionalized metal immobilized magnetic nanoparticles for highly selective and sensitive enrichment of phosphopeptides.
Synthesis of adenosine functionalized metal immobilized magnetic nanoparticles for highly selective and sensitive enrichment of phosphopeptides.
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DOI：
10.1039/c2cc31641b
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发表时间：
2012-05
影响因子：
4.9
通讯作者：
Liyuan Zhang;Qun Zhao;Zhen Liang;Kaiguang Yang;Liangliang Sun;L. Zhang;Yukui Zhang
中科院分区：
文献类型：
--
作者：
Liyuan Zhang;Qun Zhao;Zhen Liang;Kaiguang Yang;Liangliang Sun;L. Zhang;Yukui Zhang
合成了一种新型的以三磷酸腺苷为螯合配体的IMAC材料，并将其应用于捕集磷酸肽。该方法首次实现了非磷肽在5000倍稀释下的选择性和3amol的靶向富集度。
A new type of IMAC material, with ATP as the chelating ligand, was synthesized and applied to capture phosphopeptides. For the first time, the approach for phosphopeptide enrichment could provide selectivity under 5000-fold dilution by nonphosphopeptides, and sensitivity of on-target enrichment at 3 amol.