Pressure dependence of pyrene excimer fluorescence in human erythrocyte membranes.
Pressure dependence of pyrene excimer fluorescence in human erythrocyte membranes.
复制标题
人红细胞膜中芘准分子荧光的压力依赖性。
DOI：
10.1016/0005-2736(82)90175-4
复制
发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Schachter,D
中科院分区：
文献类型：
--
作者：
Flamm,M;Okubo,T;Turro,NJ;Schachter,D
The intensity of pyrene excimer fluorescence in human erythrocyte membranes and in sonicated dispersions of the membrane lipid (liposomes) was examined as a function of pressure (1–2080 bar) and temperature (5–40°C). Higher pressure or lower temperature decreased the excimer/monomer intensity ratios. A thermotropic transition was detected in both membranes and liposomes by plots of the logarithm of the excimer/monomer intensity ratio versus 1/K. The transition temperature of the membranes was 19–21°C at 1 bar and 28–31°C at 450 bar, a shift with pressure of approx. 20–22 K per kbar. Corresponding transition temperatures of the liposomes were 21°C at 1 bar and 33°C at 450 bar, a shift of approx. 27 K per kbar. The observed pressure dependence of the thermotropic transition temperature is similar to that reported for phospholipid bilayers and greatly exceeds that of protein conformation changes. In concert with the liposome studies the results provide direct evidence for a lipid transition in the erythrocyte membrane.
