Preparation, characterization and in vitro biological study of biomimetic three-dimensional gelatin-montmorillonite/cellulose scaffold for tissue engineering
Preparation, characterization and in vitro biological study of biomimetic three-dimensional gelatin-montmorillonite/cellulose scaffold for tissue engineering
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DOI：
10.1007/s10856-009-3818-x
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发表时间：
2009-12-01
影响因子：
3.7
通讯作者：
Harding, David R. K.
中科院分区：
文献类型：
--
作者：
Haroun, Ahmed A.;Gamal-Eldeen, Amira;Harding, David R. K.
研究了明胶（Gel）与纤维素（Cel）在蒙脱土（MMT）存在下的共混对凝胶溶胀行为、体外降解及表面形貌的影响。此外，所制备的生物复合材料对人骨肉瘤细胞（Saos-2）的特性的影响，包括增殖、支架/细胞相互作用、细胞凋亡及其诱导成骨和分化的潜能。以戊二醛（GA）和N，N-亚甲基双丙烯酰胺（MBA）为交联剂，采用插层法和冷冻干燥法制备了交联生物复合材料。研究了其SEM形貌、X射线衍射表征和体外生物降解性能。结合Saos-2细胞的3-D仿生多孔支架的成功产生表明其利用细胞-基质相互作用的从头骨形成的潜力。体外研究表明，含12%和6%的MMT，5%和0.5%的GA交联的支架是两种最有效的可生物降解支架，分别促进Saos-2细胞的增殖、迁移、扩增、粘附、渗透、铺展和分化。MMT改善了成骨细胞与生物复合材料之间的细胞相容性。体外生物相容性试验表明，该支架具有良好的生物相容性，是一种新型的组织工程生物复合材料。
This work focused on studying the effect of blending gelatin (Gel) with Cellulose (Cel), in the presence of montmorillonite (MMT), on the swelling behavior, in vitro degradation and surface morphology. Additionally, the effect of the prepared biocomposites on the characteristics of the human osteosarcoma cells (Saos-2), including proliferation, scaffold/cells interactions, apoptosis and their potential of the cells to induce osteogenesis and differentiation was evaluated. The crosslinked biocomposites with glutaraldehyde (GA) or N,N-methylene-bisacrylamide (MBA) was prepared via an intercalation process and freeze-drying technique. Properties including SEM morphology, X-ray diffraction characterization and in vitro biodegradation were investigated. The successful generation of 3-D biomimetic porous scaffolds incorporating Saos-2 cells indicated their potential for de novo bone formation that exploits cell-matrix interactions. In vitro studies revealed that the scaffolds containing 12 and 6% MMT crosslinked by 5 and 0.5% GA seem to be the two most efficient and effective biodegradable scaffolds, which promoted Saos-2 cells proliferation, migration, expansion, adhesion, penetration, spreading, and differentiation, respectively. MMT improved cytocompatibility between the osteoblasts and the biocomposite. In vitro analysis indicated good biocompatibility of the scaffold and presents the scaffold as a new potential candidate as suitable biohybrid material for tissue engineering.