Tudor-related proteins TDRD1/MTR-1, TDRD6 and TDRD7/TRAP: Domain composition, intracellular localization, and function in male germ cells in mice
Tudor-related proteins TDRD1/MTR-1, TDRD6 and TDRD7/TRAP: Domain composition, intracellular localization, and function in male germ cells in mice
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DOI：
10.1016/j.ydbio.2006.10.046
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发表时间：
2007-01-01
影响因子：
2.7
通讯作者：
Nakatsuji, Norio
中科院分区：
文献类型：
--
作者：
Hosokawa, Mihoko;Shoji, Masanobu;Nakatsuji, Norio
许多动物的生殖系细胞具有一种特有的细胞质结构，称为胚粒或胚泡颗粒。在小鼠中，出生后雄性生殖细胞中突出的细胞也被称为线粒体粘固体或类色小体。TDRD 1/MTR-1含有Tudor结构域重复序列，是小鼠nuage的特异性组分，类似于果蝇Tudor，是卵母细胞和胚胎中极性颗粒/nuage的组分。我们发现，TDRD 6和TDRD 7/TRAP，其中也包含多个Tudor结构域，特异性定位于nuage和形成一个核糖核蛋白复合物与TDRD 1/MTR-1。在小鼠vasa同源物/DEAD盒多肽4（Mvh/Ddx 4）的突变体中，TDRD 1、6和7的特征性共定位被破坏，该突变体编码另一种进化上保守的nuage组分。在雄性生殖细胞分化过程中的TDRD蛋白和截短形式的体内过表达实验表明，一个单一的Tudor结构域是一个结构单位，定位或积累到nuage，但截短的，推定的显性负性形式的表达是有害的减数分裂精母细胞。这些结果表明，Tudor相关蛋白，其中包含多个重复的Tudor结构域，构成了一个进化上保守的类nuage组件在生殖系中，和他们的本地化或积累nuage可能是由一个Tudor结构域结构和下游的Mvh赋予，而该结构域的特征性重复架构是生殖细胞的分化功能是必不可少的。(c)2006年爱思唯尔公司All rights reserved.
The germ-line cells of many animals possess a characteristic cytoplasmic structure termed nuage or germinal granules. In mice, nuage that is prominent in postnatal male germ cells is also called intermitochondrial cement or chrornatoid bodies. TDRD1/MTR-1, which contains Tudor domain repeats, is a specific component of the mouse nuage, analogously to Drosophila Tudor, a constituent of polar granules/nuage in oocytes and embryos. We show that TDRD6 and TDRD7/TRAP, which also contain multiple Tudor domains, specifically localize to nuage and form a ribonucleoprotein complex together with TDRD1/MTR-1. The characteristic co-localization of TDRD1, 6 and 7 was disrupted in a mutant of mouse vasa homologue/DEAD box potypeptide 4 (Mvh/Ddx4), which encodes another evolutionarily conserved component of nuage. In vivo over-expression experiments of the TDRD proteins and truncated forms during male germ cell differentiation showed that a single Tudor domain is a structural unit that localizes or accumulates to nuage, but the expression of the truncated, putative dominant negative forms is detrimental to meiotic spermatocytes. These results indicate that the Tudor-related proteins, which contain multiple repeats of the Tudor domain, constitute an evolutionarily conserved class of nuage components in the germ-line, and their localization or accumulation to nuage is likely conferred by a Tudor domain structure and downstream of Mvh, while the characteristic repeated architecture of the domain is functionally essential for the differentiation of germ cells. (c) 2006 Elsevier Inc. All rights reserved.