Targeted DNA transfection into the mouse central nervous system using laser-induced stress waves
Targeted DNA transfection into the mouse central nervous system using laser-induced stress waves
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DOI：
10.1117/1.2128432
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发表时间：
2005-11-01
影响因子：
3.5
通讯作者：
Takishima, K
中科院分区：
文献类型：
--
作者：
Satoh, Y;Kanda, Y;Takishima, K
我们研究了应用纳秒脉冲激光诱导应力波(LISW)将基因转移到小鼠中枢神经系统(CNS)的可行性。脑室或海马区注射报告基因[增强型绿色荧光蛋白(EGFP)]，然后应用LISW，表明这种方法在新生和成年小鼠的中枢神经系统中是有效的。表达EGFP的细胞位于距组织表面至少3.5 mm处，未检测到明显的损伤。此外，EGFP的表达仅限于接触LISW的区域。采用这种方法，阳离子转移试剂聚乙烯亚胺制备的质粒DNA被证明是有效地提高了向中枢神经系统的转移效率。(C)2005年光学仪器工程师学会。
We investigated the feasibility of gene transfer into the mouse central nervous system (CNS) by applying nanosecond pulsed laser-induced stress waves (LISWs). Intraventricular or hippocampal injection of a reporter gene [enhanced green fluorescent protein (EGFP)] followed by application of LISWs showed this method to be efficient in the CNS of newborn and adult mice. Cells expressing EGFP reside at least 3.5 mm from the surface of the tissue, while no apparent damage was detected. Additionally, expression of EGFP was limited to the area that was exposed to LISWs. Using this method, the formulation of plasmid DNA by cationic transfer reagent polyethylenimine proved to be effective for improving transfer efficiency into the CNS. (C) 2005 Society of Photo-Optical Instrumentation Engineers.