Antibacterial activity of ruthenium(II) polypyridyl complex manipulated by membrane permeability and cell morphology
Antibacterial activity of ruthenium(II) polypyridyl complex manipulated by membrane permeability and cell morphology
复制标题
膜通透性和细胞形态调控钌(II)聚吡啶复合物的抗菌活性
DOI：
10.1016/j.bmcl.2015.03.090
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发表时间：
2015-05-15
影响因子：
2.7
通讯作者：
Wang, Weiyun
中科院分区：
文献类型：
--
作者：
Sun, Dongdong;Zhang, Weiwei;Wang, Weiyun
本研究探讨了钌（II）配合物RuBP的抗菌效果和RuBP对细菌的作用机制。结果表明，RuBP对金黄色葡萄球菌、四属微球菌等革兰氏阳性菌有明显的抑制作用。细胞摄取和激光共聚焦显微镜研究揭示了四属微球菌细胞对RuBP的有效摄取。四属微球菌和S.用RuBP处理的金黄色葡萄球菌进一步证实了两种细菌与RuBP的直接接触会损害细胞膜和膜完整性，这可能最终诱导生长抑制和细菌死亡。RuBP处理后，细菌悬浮液的电导率增加。光谱研究和琼脂糖凝胶电泳表明，RuBP处理的细菌细胞中完整的DNA和RNA减少或消失，从而证明RuBP通过增加细胞膜的渗透性来执行其抗菌功能。该研究为了解RuBP的抗菌作用和设计用于其他生物医学应用的金属配合物抗生素提供了新的见解。(C)2015爱思唯尔有限公司版权所有。
This study investigates the antibacterial effects of the ruthenium(II) complex RuBP and the mechanism of RuBP action on bacteria. Results show that RuBP can inhibit the growth of Gram-positive bacteria, such as Staphylococcus aureus and Micrococcus tetragenus. Cellular uptake and laser confocal microscopic studies reveal the efficient uptake of RuBP by Micrococcus tetragenus cells. Scanning electron microscopic observations of the morphologies of Micrococcus tetragenus and S. aureus treated with RuBP further confirm that direct contact of both bacteria with RuBP can damage the cell membrane and membrane integrity, which may eventually induce growth inhibition and bacterial death. After RuBP treatment, the electrical conductivity of the bacterial suspensions increases. Spectroscopic studies and agarose gel electrophoresis indicate that intact DNA and RNA decrease or disappear in RuBP-treated bacterial cells, thus demonstrating that RuBP performs its antibacterial function by increasing the permeability of cell membranes. This study provides new insights for understanding the antibacterial actions of RuBP and designing metal complex antibiotics for other biomedical applications. (C) 2015 Elsevier Ltd. All rights reserved.