METHOD FOR ISOLATION OF INTACT ISLETS OF LANGERHANS FROM RAT PANCREAS
METHOD FOR ISOLATION OF INTACT ISLETS OF LANGERHANS FROM RAT PANCREAS
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DOI：
10.2337/diab.16.1.35
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发表时间：
1967-01-01
期刊：
影响因子：
7.7
通讯作者：
KOSTIANOVSKY, M
中科院分区：
文献类型：
--
作者：
LACY, PE;KOSTIANOVSKY, M
本文介绍一种从正常大鼠胰腺分离完整胰岛的简单方法。该方法基于通过将Hanks溶液注射到胰管系统中，然后将胰腺在胶原酶中孵育来破坏腺泡实质。胰岛可以通过沉淀从这种混合物中迅速分离出来。分离的胰岛在体外释放胰岛素，孵育后通过光镜和电镜观察显示正常。还描述了用于分离大量胰岛用于生化研究的离心方法。
A simple method for the isolation of intact islets from the normal rat pancreas is described. The method is based upon disruption of the acinar parenchyma by injecting Hanks solution into the pancreatic duct system followed by incubation of the pancreas in collagenase. Islets can be separated rapidly from this mixture by sedimentation. The isolated islets release insulin in vitro and appear normal by light and electron microscopy after incubation. A centrifugation method is also described for isolation of large quantities of islets for biochemical studies.