High throughput 3D super-resolution microscopy reveals Caulobacter crescentus in vivo Z-ring organization
High throughput 3D super-resolution microscopy reveals Caulobacter crescentus in vivo Z-ring organization
复制标题
DOI：
10.1073/pnas.1313368111
复制
发表时间：
2014-03-25
影响因子：
11.1
通讯作者：
Manley, Suliana
中科院分区：
文献类型：
--
作者：
Holden, Seamus J.;Pengo, Thomas;Manley, Suliana
我们创建了一种高通量的光活化定位显微镜（PALM）模式，可以在细胞周期的所有阶段对数百种同步细菌进行自动3D PALM成像。我们使用高通量PALM来研究活新月柄杆菌中细菌细胞分裂蛋白FtsZ的纳米级组织。我们观察到，FtsZ主要定位为一个斑片状的中细胞带，只有很少作为一个连续的环，支持模型的“Z-环”的组织，FtsZ原丝随机分布在带内，只有弱相互作用。我们发现了一个以前未确定的时期的快速环收缩的细胞周期的最后阶段的证据。我们还发现，DNA损伤导致高密度连续Z环的产生，这可能会阻碍胞质分裂。我们的研究结果提供了一个详细的定量图片在体内Z环组织。
We created a high-throughput modality of photoactivated localization microscopy (PALM) that enables automated 3D PALM imaging of hundreds of synchronized bacteria during all stages of the cell cycle. We used high-throughput PALM to investigate the nanoscale organization of the bacterial cell division protein FtsZ in live Caulobacter crescentus. We observed that FtsZ predominantly localizes as a patchy midcell band, and only rarely as a continuous ring, supporting a model of "Z-ring" organization whereby FtsZ protofilaments are randomly distributed within the band and interact only weakly. We found evidence for a previously unidentified period of rapid ring contraction in the final stages of the cell cycle. We also found that DNA damage resulted in production of high-density continuous Z-rings, which may obstruct cytokinesis. Our results provide a detailed quantitative picture of in vivo Z-ring organization.