A quantitative view of the transcriptome of Schistosoma mansoni adult-worms using SAGE.
A quantitative view of the transcriptome of Schistosoma mansoni adult-worms using SAGE.
复制标题
使用鼠尾草的成年人瘤的转录组的定量观点。
DOI：
10.1186/1471-2164-8-186
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发表时间：
2007-06-21
期刊：
影响因子：
4.4
通讯作者：
Dias-Neto, Emmanuel
中科院分区：
文献类型：
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作者：
Ojopi, Elida P B;Oliveira, Paulo S L;Nunes, Diana N;Paquola, Apua;DeMarco, Ricardo;Gregorio, Sheila P;Aires, Karina A;Menck, Carlos F M;Leite, Luciana C C;Verjovski-Almeida, Sergio;Dias-Neto, Emmanuel
血吸虫属的五种寄生吸虫扁形虫是血吸虫病的病原体，血吸虫病是一种在许多发展中国家流行的疾病，影响着世界各地数百万患者。通过使用SAGE（基因表达系列分析），我们在这里描述了曼氏血吸虫转录组的第一次大规模定量分析，这是该属中流行病学最相关的物种之一。从混合的雄性和雌性成虫中提取mRNA后，构建SAGE文库并测序，产生68，238个标签，覆盖在该发育阶段表达的6，000多个基因。对有序标签列表的分析显示，F10蛋壳蛋白、pol-多聚蛋白、HSP 86、14-3-3和尚未鉴定的转录物的基因是汇集的蠕虫中最丰富的五个基因。而在蠕虫中发现的100个最丰富的标签中，只有8%的标签被标记。mansoni不能归属于该寄生虫的转录本，46.9%的总双标签不能被定位，表明大多数最稀有的转录本的3个序列仍有待鉴定。将我们的SAGE标签映射到S。mansoni基因提示至少13个基因转录本中存在交替多聚腺苷酸化。这些事件中的大多数似乎缩短了mRNA的3个UTR，这可能对它们的稳定性和调节产生影响。SAGE结果显示，大部分S. mansoni基因。转录组数据表明，替代聚腺苷酸化可能用于控制这种生物体中mRNA的稳定性。当转录组与可用的蛋白质组数据进行比较时，我们观察到约50%的相关性，这表明转录和转录后调控对于确定S. mansoni从其他生命周期阶段产生的SAGE标签应有助于揭示这种重要寄生虫的基因表达动态。
Five species of the genus Schistosoma, a parasitic trematode flatworm, are causative agents of Schistosomiasis, a disease that is endemic in a large number of developing countries, affecting millions of patients around the world. By using SAGE (Serial Analysis of Gene Expression) we describe here the first large-scale quantitative analysis of the Schistosoma mansoni transcriptome, one of the most epidemiologically relevant species of this genus. After extracting mRNA from pooled male and female adult-worms, a SAGE library was constructed and sequenced, generating 68,238 tags that covered more than 6,000 genes expressed in this developmental stage. An analysis of the ordered tag-list shows the genes of F10 eggshell protein, pol-polyprotein, HSP86, 14-3-3 and a transcript yet to be identified to be the five top most abundant genes in pooled adult worms. Whereas only 8% of the 100 most abundant tags found in adult worms of S. mansoni could not be assigned to transcripts of this parasite, 46.9% of the total ditags could not be mapped, demonstrating that the 3 sequence of most of the rarest transcripts are still to be identified. Mapping of our SAGE tags to S. mansoni genes suggested the occurrence of alternative-polyadenylation in at least 13 gene transcripts. Most of these events seem to shorten the 3 UTR of the mRNAs, which may have consequences over their stability and regulation. SAGE revealed the frequency of expression of the majority of the S. mansoni genes. Transcriptome data suggests that alternative polyadenylation is likely to be used in the control of mRNA stability in this organism. When transcriptome was compared with the proteomic data available, we observed a correlation of about 50%, suggesting that both transcriptional and post-transcriptional regulation are important for determining protein abundance in S. mansoni. The generation of SAGE tags from other life-cycle stages should contribute to reveal the dynamics of gene expression in this important parasite.