Molecular Cloning and Functional Characterization of Two Brachypodium distachyon UBC13 Genes Whose Products Promote K63-Linked Polyubiquitination.
Molecular Cloning and Functional Characterization of Two Brachypodium distachyon UBC13 Genes Whose Products Promote K63-Linked Polyubiquitination.
复制标题
两个二穗短柄草 UBC13 基因的分子克隆和功能表征，其产物促进 K63 连接的多泛素化
DOI：
10.3389/fpls.2015.01222
复制
发表时间：
2015
影响因子：
5.6
通讯作者：
Xiao W
中科院分区：
文献类型：
--
作者：
Guo H;Wen R;Liu Z;Datla R;Xiao W
生物体不断受到来自环境来源的DNA损伤。由于植物的固着性，紫外线照射是一种主要的遗传毒性剂，对植物的存活、基因组稳定性和作物产量构成重大威胁。此外，其他环境化学物质也会影响植物基因组的稳定性。真核生物已经进化出一种机制来科普复制阻断病变并稳定基因组。这种机制被称为无错DNA损伤耐受，并由K63连接的PCNA多聚泛素化介导。K63-linked polyubiquitination相关基因最近已经从模式植物如拟南芥和水稻中分离出来，但我们不知道在作物模式二穗短柄草中有这样的报道。在这里，我们报告的鉴定和功能特性的两个B。二穗草UBC 13基因。这两个Ubc 13与来自其他物种的Uevs形成异二聚体，其能够在体外催化K63聚泛素化。这两个基因都可以在功能上拯救酵母ubc 13无效突变体免受DNA损伤剂的杀伤。这些结果表明Ubc 13-Uev促进的K63连接的多泛素化在包括B在内的真核生物中是高度保守的。二穗子与最近的研究结果一致，K63连接的多聚泛素化参与了几个发展和应激反应途径，BdUbc 13的表达似乎是组成性的，并受到异常温度的调节。
Living organisms are constantly subject to DNA damage from environmental sources. Due to the sessile nature of plants, UV irradiation is a major genotoxic agent and imposes a significant threat on plant survival, genome stability and crop yield. In addition, other environmental chemicals can also influence the stability of the plant genome. Eukaryotic organisms have evolved a mechanism to cope with replication-blocking lesions and stabilize the genome. This mechanism is known as error-free DNA damage tolerance, and is mediated by K63-linked PCNA polyubiquitination. Genes related to K63-linked polyubiquitination have been isolated recently from model plants like Arabidopsis and rice, but we are unaware of such reports on the crop model Brachypodium distachyon. Here, we report the identification and functional characterization of two B. distachyon UBC13 genes. Both Ubc13s form heterodimers with Uevs from other species, which are capable of catalyzing K63 polyubiquitination in vitro. Both genes can functionally rescue the yeast ubc13 null mutant from killing by DNA-damaging agents. These results suggest that Ubc13-Uev-promoted K63-linked polyubiquitination is highly conserved in eukaryotes including B. distachyon. Consistent with recent findings that K63-linked polyubiquitination is involved in several developmental and stress-responsive pathways, the expression of BdUbc13s appears to be constitutive and is regulated by abnormal temperatures.