Characterization of the Monomer and Dimer of Tobacco Mosaic Virus by Transient Electric Birefringence1
Characterization of the Monomer and Dimer of Tobacco Mosaic Virus by Transient Electric Birefringence1
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通过瞬态电双折射表征烟草花叶病毒的单体和二聚体1
DOI：
10.1021/ja01596a013
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发表时间：
1956
影响因子：
15
通讯作者：
A. Haltner
中科院分区：
文献类型：
--
作者：
C. T. O'konski;A. Haltner
通过对若干制剂的瞬态电双折射测量，发现烟草花叶病毒单体单元在稀水溶液中的旋转扩散常数为333秒-1。对应的长度为3416±50a，明显大于2980±10 Á。在最近的电子显微镜研究中报道。假设一个紧凑的圆棒，3416 a长，并采用X射线直径和测量的比容，分子量为50 X 106，与一个独立的精确方法的结果一致。新的长度值将参考其他类型的测量数据进行讨论。证据表明存在端到端二聚体在所有的准备，但一个。由于细长大分子围绕其短轴的旋转扩散常数最关键地取决于长度，因此瞬态电双折射是测量刚性大分子长度的一种特别敏感的方法。介绍了改进的实验方法。
The rotational diffusion constant of the monomer unit of tobacco mosaic virus in dilute aqueous solution was found to be 333 sec.-1 from transient electric birefringence measurements of a number of preparations. This value corresponds to a length of 3416±50 A., which is significantly greater than 2980±10 Á., reported in the most recent electron microscope study. Assuming a compact circular rod, 3416 A. long, and employing the X-ray diameter and the measured specific volume, the molecular weight is 50 X 106, in agreement with the result of an independent precise method. The new value for the length is discussed with reference to data from other types of measurements. Evidence was obtained for the existence of an end-to-end dimer in all but one of the preparations. Because theconstant for rotational diffusion of an elongated macro-molecule about its short axis depends most critically upon the length, transient electric birefringence is an especially sensitive method for measuring the lengths of rigid macromolecules. Improved experimental methods are described.