Schwann Cell Phenotype Changes in Aging Human Dental Pulp
Schwann Cell Phenotype Changes in Aging Human Dental Pulp
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DOI：
10.1177/0022034517733967
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发表时间：
2018-03-01
影响因子：
7.6
通讯作者：
Schmachtenberg, O.
中科院分区：
文献类型：
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作者：
Couve, E.;Lovera, M.;Schmachtenberg, O.
雪旺细胞是支持周围神经系统中轴突发育、维持、防御和再生的胶质细胞。关于成年恒牙牙髓中许旺细胞的组织、可塑性和老化的知识有限。本研究试图将年轻和老年成年牙齿之间的雪旺细胞表型模式的变化与牙髓的神经元，免疫和血管成分联系起来。雪旺细胞在牙本质牙髓界面形成一个突出的神经胶质网络，包括非髓鞘形成和髓鞘形成表型，与神经纤维和树突状细胞形成多细胞神经免疫界面。雪旺细胞表型通过S100、胶质细胞酸性蛋白（GFAP）、髓鞘碱性蛋白（MBP）、Sox 10、GAP 43和p75 NTR标记物的表达来识别。在年轻的成年牙齿中，密集的非髓鞘形成的雪旺氏细胞通过成牙本质细胞层与感觉神经末梢密切相关，到达相邻的前牙本质/牙本质域。虽然GAP 43和p75 NTR在年轻成人牙齿的非髓鞘形成雪旺细胞中高度表达，但这些标记物在老年成人牙齿中的存在显着下降。髓鞘化轴突，识别MBP表达，主要存在于Raschkow丛和牙髓中的神经束内，但它们的密度显着降低，在老年人与年轻的成年人牙齿。这些数据揭示了牙髓神经胶质网络内与年龄相关的变化，与老年人与年轻人牙齿中冠状牙髓神经支配的减少有关。雪旺细胞作为细胞组分在牙本质牙髓界面的突出支持了这样的观点，即它们与感觉神经末梢和免疫系统组分的关联形成了用于防御病原体和牙本质修复的综合多细胞屏障的一部分。
Schwann cells are glial cells that support axonal development, maintenance, defense, and regeneration in the peripheral nervous system. There is limited knowledge regarding the organization, plasticity, and aging of Schwann cells within the dental pulp in adult permanent teeth. The present study sought to relate changes in the pattern of Schwann cell phenotypes between young and old adult teeth with neuronal, immune, and vascular components of the dental pulp. Schwann cells are shown to form a prominent glial network at the dentinpulp interface, consisting of nonmyelinating and myelinating phenotypes, forming a multicellular neuroimmune interface in association with nerve fibers and dendritic cells. Schwann cell phenotypes are recognized by the expression of S100, glial fibrillary acidic protein (GFAP), myelin basic protein (MBP), Sox10, GAP43, and p75NTR markers. In young adult teeth, a dense population of nonmyelinating Schwann cells projects processes in close association with sensory nerve terminals through the odontoblast layer, reaching the adjacent predentin/dentin domain. While GAP43 and p75NTR are highly expressed in nonmyelinating Schwann cells from young adult teeth, the presence of these markers declines significantly in old adult teeth. Myelinated axons, identified by MBP expression, are mainly present at the Raschkow plexus and within nerve bundles in the dental pulp, but their density is significantly reduced in old adult versus young adult teeth. These data reveal age-related changes within the glial network of the dental pulp, in association with a reduction of coronal dental pulp innervation in old adult versus young adult teeth. The prominence of Schwann cells as a cellular component at the dentinpulp interface supports the notion that their association with sensory nerve terminals and immune system components forms part of an integrated multicellular barrier for defense against pathogens and dentin repair.