Diploid ancestry and evolution of Triticum kotschyi and T. peregrinum examined using variation in repeated nucleotide sequences
Diploid ancestry and evolution of Triticum kotschyi and T. peregrinum examined using variation in repeated nucleotide sequences
复制标题
使用重复核苷酸序列的变异检查Triticum kotschyi 和 T. peregrinum 的二倍体祖先和进化
作者：
Hong;J. Dvorak;J. Waines
Triticum peregrinum Hackel（syn.易变山羊草（Aegilops variabilis Eig）和T. kotschyi（Boiss.）Bowden（syn. AE. kotschyi Boiss.）是非常接近的异源四倍体物种。先前的研究表明，它们具有一对来自二倍体T的U基因组。umbellulatum（Zhuk.）Hackel和一对S染色体组。为了重新研究它们基因组的起源，我们在与27个克隆的重复核苷酸序列和一个5S rRNA基因克隆杂交的Southern印迹中鉴定了小麦属所有二倍体物种的诊断带。感觉上是鲍登。然后在两个四倍体物种中确定每个诊断带的存在。两个四倍体物种中的一对基因组被发现与T的U基因组几乎相同。umbellulatum和T. longissimum（Schweif. et Muschl.）Bowden或T. sharonense（Eig）Waines &约翰逊（syn. ... class ='class3'>或小麦属系统发育树中紧接在亚枝节之前的节间.
Triticum peregrinum Hackel (syn. Aegilops variabilis Eig) and T. kotschyi (Boiss.) Bowden (syn. Ae. kotschyi Boiss.) are closely related allotetraploid species. Previous research indicated that they have a pair of U genomes from diploid T. umbellulatum (Zhuk.) Hackel and a pair of S genomes from a diploid species of Triticum section Sitopsis. To reinvestigate the origin of their genomes, we identified diagnostic bands in Southern blots hybridized with 27 cloned repeated nucleotide sequences and one 5S rRNA gene clone for all diploid species of Triticum L. sensu Bowden. The presence of each diagnostic band was then determined in the two tetraploid species. One pair of the genomes in both tetraploid species was found to be virtually identical to the U genome of T. umbellulatum and the other to the S1 genome of T. longissimum (Schweif. et Muschl.) Bowden or T. sharonense (Eig) Waines &Johnson (syn. Aegilops sharonensis Eig) or the internode in the phylogenetic tree of Triticum immediately preceding the diver...