Post-hatching developmental changes in the ultrastructure of the duodenal absorptive epithelial cells in 1, 10 and 60-d-old chickens, with special reference to mitochondria.
Post-hatching developmental changes in the ultrastructure of the duodenal absorptive epithelial cells in 1, 10 and 60-d-old chickens, with special reference to mitochondria.
复制标题
1、10 和 60 日龄鸡的十二指肠吸收上皮细胞超微结构的孵化后发育变化，特别是线粒体。
DOI：
10.1080/00071669208417487
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发表时间：
1992
影响因子：
2
通讯作者：
Y. Isshiki
中科院分区：
文献类型：
--
作者：
K. Yamauchi;S. Iida;Y. Isshiki
1.本研究观察了1、10和60日龄白色来航鸡（WL）和肉仔鸡（BR）十二指肠吸收上皮细胞超微结构、细胞面积和组织学RNA含量的发育变化，特别是线粒体的发育变化。2. 1日龄WL和BR的上皮细胞在细胞表面附近和核下区域有发达的高尔基区和密集的杆状Mt簇。BR的内质网发达，但核上空泡较少，核上空泡内有大量游离核糖体，有的线粒体呈芽状突起（蝌蚪型）。这表明WL的上皮细胞的精细结构成熟参与了细胞成熟的过程，而BR的上皮细胞在孵化时已基本成熟。3.在10日龄WL和BR，核上空泡已消失，Mt的数量增加。在WL中，除了游离核糖体减少外，Mt发育为蝌蚪型，并进一步发育为哑铃型。在BR中，Mt也聚集在核周区，其中一些已经从哑铃形发展到厚甜甜圈型。这些研究结果表明，在这两个品种的上皮细胞是更发达的超微结构比那些在1天的老，BR的上皮细胞比WL的消化和吸收功能更活跃。4. 60日龄WL和BR的Mt类型多样，基质较10日龄的薄，表明细胞结构和功能已达到稳定状态。5.各日龄BR的细胞面积和RNA含量均高于WL，RNA含量均在10日龄达到最大值，60日龄次之。6. MT形态由杆状向蝌蚪型转变。后者则由哑铃型向甜甜圈型发展，与上皮细胞功能增强有关。7.因此，BR的上皮细胞在每个年龄比WL细胞更高度活化，并在10 d左右达到形态和生理成熟。
1. Post-hatching developmental changes in the ultrastructure, cell area and histological RNA content of duodenal absorptive epithelial cells were observed in 1, 10 and 60-d-old White Leghorn (WL) and broiler (BR) chickens, with special reference to the mitochondria (Mt). 2. Epithelial cells in 1-d-old WL and BR had a well-developed Golgi area and a dense cluster of rod type Mt near the cell surface and at the infranuclear region. In BR, cells included well developed profiles of endoplasmic reticulum but fewer supranuclear vacuoles than those of WL, in which numerous free ribosomes were also found. Some Mt in BR showed a bud-like protrusion from the main body (tadpole type Mt). These suggest that the fine structural maturation of the epithelial cells in WL is involved in the process of cell maturation but epithelial cells of BR have almost matured at hatching. 3. In 10-d-old WL and BR, supranuclear vacuoles had disappeared and Mt increased in number. In WL, in addition to a decrease in free ribosomes, Mt developed to the tadpole type and further to dumbbell type ones. In BR, Mt also aggregated at the perinuclear region and some of them had developed from dumbbell shapes to a thick doughnut type. These findings indicate that epithelial cells in both breeds are more developed ultrastructurally than those in 1-d-old and that epithelial cells in BR are more activated for digestive and absorptive functions than those in WL. 4. In 60-d-old WL and BR, Mt showed various types with thinner matrices than 10-d-old, suggesting that the cell structure and function had reached a stable state. 5. BR revealed higher values for cell area and RNA content than WL at every age and RNA content of both breeds were maximal at 10 d, followed by 60 d. 6. Mt changed their shapes from rod type to tadpole type. The latter then developed to doughnut type via dumbbell type, related to an increase of epithelial cell functions. 7. Epithelial cells of BR are thus more highly activated than WL cells at each age and reach morphological and physiological maturation around 10 d.