Regular and moderate exercise before experimental sepsis reduces the risk of lung and distal organ injury
Regular and moderate exercise before experimental sepsis reduces the risk of lung and distal organ injury
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DOI：
10.1152/japplphysiol.01061.2011
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发表时间：
2012-04-01
影响因子：
3.3
通讯作者：
Rocco, Patricia R. M.
中科院分区：
文献类型：
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作者：
de Araujo, Carla C.;Silva, Johnatas D.;Rocco, Patricia R. M.
de Araujo CC，Silva JD，Samary CS，Guimaraes IH，Marques PS，Oliveira GP，do Carmo LGRR，Goldenberg RC，Bakker-Abreu I，迪亚兹BL，罗查NN，Capelozzi VL，Pelosi P，Rocco PRM.实验性脓毒症前进行规律和适度的运动可降低肺和远端器官损伤的风险。J Appl Physiol 112：1206-1214，2012.首次发表于2012年1月19日; doi：10.1152/japplphysiol.01061.2011。身体活动调节炎症和免疫反应在正常和病理条件。我们研究了在诱导实验性脓毒症前进行规律和适度的运动是否能降低肺和远端器官损伤的风险和生存率。将124只BALB/c小鼠随机分为两组：久坐组（S）和训练组（T）。T组动物在电动跑台上跑，中等强度，5%分级，30 min/d，每周3次，共8 wk。采用超声心动图评价心脏运动适应性。与S组相比，T组左室重量和左室收缩容积增加。T组和S组进一步随机分为盲肠结扎穿孔术（CLP）组和假手术组（对照组）。24 h后，测定肺力学和组织学，肺、心、肾、肝和小肠绒毛中细胞凋亡的程度，以及白细胞介素（IL）-6，KC（IL-8小鼠功能同系物），IL-1 β，IL-10，支气管肺泡灌洗液（BALF）和腹腔灌洗液（PLF）中的细胞数以及血浆。在CLP中，与S组相比，T组显示：1）生存率提高; 2）肺静态弹性、肺泡塌陷、胶原蛋白和弹性纤维含量、BALF、PLF和血浆中中性粒细胞数量以及肺和远端器官细胞凋亡减少; 3）BALF和血浆中IL-10增加，PLF中IL-6、KC和IL-1 β减少。总之，在诱导脓毒症之前进行有规律的适度运动可以降低肺和远端器官损伤的风险，从而提高生存率。
de Araujo CC, Silva JD, Samary CS, Guimaraes IH, Marques PS, Oliveira GP, do Carmo LGRR, Goldenberg RC, Bakker-Abreu I, Diaz BL, Rocha NN, Capelozzi VL, Pelosi P, Rocco PRM. Regular and moderate exercise before experimental sepsis reduces the risk of lung and distal organ injury. J Appl Physiol 112: 1206-1214, 2012. First published January 19, 2012; doi:10.1152/japplphysiol.01061.2011.-Physical activity modulates inflammation and immune response in both normal and pathologic conditions. We investigated whether regular and moderate exercise before the induction of experimental sepsis reduces the risk of lung and distal organ injury and survival. One hundred twenty-four BALB/c mice were randomly assigned to two groups: sedentary (S) and trained (T). Animals in T group ran on a motorized treadmill, at moderate intensity, 5% grade, 30 min/day, 3 times a week for 8 wk. Cardiac adaptation to exercise was evaluated using echocardiography. Systolic volume and left ventricular mass were increased in T compared with S group. Both T and S groups were further randomized either to sepsis induced by cecal ligation and puncture surgery (CLP) or sham operation (control). After 24 h, lung mechanics and histology, the degree of cell apoptosis in lung, heart, kidney, liver, and small intestine villi, and interleukin (IL)-6, KC (IL-8 murine functional homolog), IL-1 beta, IL-10, and number of cells in bronchoalveolar lavage (BALF) and peritoneal lavage (PLF) fluids as well as plasma were measured. In CLP, T compared with S groups showed: 1) improvement in survival; 2) reduced lung static elastance, alveolar collapse, collagen and elastic fiber content, number of neutrophils in BALF, PLF, and plasma, as well as lung and distal organ cell apoptosis; and 3) increased IL-10 in BALF and plasma, with reduced IL-6, KC, and IL-1 beta in PLF. In conclusion, regular and moderate exercise before the induction of sepsis reduced the risk of lung and distal organ damage, thus increasing survival.