BZR1 is a transcriptional repressor with dual roles in brassinosteroid homeostasis and growth responses
BZR1 is a transcriptional repressor with dual roles in brassinosteroid homeostasis and growth responses
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DOI：
10.1126/science.1107580
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发表时间：
2005-03-11
期刊：
影响因子：
56.9
通讯作者：
Wang, ZY
中科院分区：
文献类型：
--
作者：
He, JX;Gendron, JM;Wang, ZY
油菜素内酯（BR）的稳态和信号传导对植物的正常生长和发育至关重要。通过细胞表面受体激酶和细胞内组分的BR信号传导导致核蛋白BZR1的去磷酸化和积累。然而，BR信号传导如何调节基因表达仍然未知。在此我们表明，BZR1是一种转录抑制因子，它具有一个以前未知的DNA结合结构域，并直接与反馈调节的BR生物合成基因的启动子结合。基因芯片分析确定了BZR1的其他潜在靶点，并且与生理学研究一起表明，BZR1通过在调节BR生物合成和下游生长反应中发挥双重作用来协调BR稳态和信号传导。
Brassinosteroid (BR) homeostasis and signaling are crucial for normal growth and development of plants. BR signaling through cell-surface receptor kinases and intracellular components leads to dephosphorylation and accumulation of the nuclear protein BZR1. How BR signaling regulates gene expression, however, remains unknown. Here we show that BZR1 is a transcriptional repressor that has a previously unknown DNA binding domain and binds directly to the promoters of feed back-regulated BR biosynthetic genes. Microarray analyses identified additional potential targets of BZR1 and illustrated, together with physiological studies, that BZR1 coordinates BR homeostasis and signaling by playing dual roles in regulating BR biosynthesis and downstream growth responses.