Immunization via hair follicles by topical application of naked DNA to normal skin
Immunization via hair follicles by topical application of naked DNA to normal skin
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DOI：
10.1038/12856
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发表时间：
1999-09-01
影响因子：
46.9
通讯作者：
Khavari, PA
中科院分区：
文献类型：
--
作者：
Fan, HR;Lin, Q;Khavari, PA
为了测试对皮肤中少量外源蛋白产生的免疫反应，我们将裸DNA水溶液应用于未经处理的正常皮肤。局部应用lacZ和乙型肝炎B表面抗原（HBsAg）的质粒表达载体至完整皮肤诱导显示T-H2特征的抗原特异性免疫应答。对于HBsAg，特异性抗体和细胞应答被诱导至与通过肌内注射可商购的重组HBsAg多肽疫苗产生的应答相同的数量级。最后，局部基因转移依赖于正常毛囊的存在。
In order to test the immune response generated to small amounts of foreign protein in skin, we applied naked DNA in aqueous solution to untreated normal skin. Topical application of plasmid expression vectors for lacZ and the hepatitis B surface antigen (HBsAg) to intact skin induced antigen-specific immune responses that displayed T-H2 features. For HBsAg, specific antibody and cellular responses were induced to the same order of magnitude as those produced by intramuscular injection of the commercially available recombinant HBsAS polypeptide vaccine. Finally, topical gene transfer was dependent on the presence of normal hair follicles.