Tissue Specific Diurnal Rhythms of Metabolites and Their Regulation during Herbivore Attack in a Native Tobacco, Nicotiana attenuata
Tissue Specific Diurnal Rhythms of Metabolites and Their Regulation during Herbivore Attack in a Native Tobacco, Nicotiana attenuata
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DOI：
10.1371/journal.pone.0026214
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发表时间：
2011-10-18
期刊：
影响因子：
3.7
通讯作者：
Baldwin, Ian T.
中科院分区：
文献类型：
--
作者：
Kim, Sang-Gyu;Yon, Felipe;Baldwin, Ian T.
生态性能是所有关于时间和内源性时钟，允许夹带的节奏和预期的健身决定事件正在迅速的特点。植物如何预测每日的非生物胁迫，如清晨的寒冷和中午的干旱，以及生物胁迫，如病原体感染的时间，正在探索中，但对生物钟在调节对昆虫食草动物和互利主义者的反应中的作用知之甚少，已知其行为受到强烈的昼夜调节，其攻击被称为重新配置植物代谢组。我们开发了一个液相色谱-质谱分析程序，并分析其输出与基于模型的峰值采摘算法，以确定代谢物的昼夜积累模式，在库/源叶和根在一个公正的方式。强昼夜模式的代谢产物模拟攻击的专业草食动物，天蛾幼虫的反应进行了分析和注释与内部和公共数据库。根和叶有很大的不同的节奏，只有10个离子的182振荡离子在叶和179振荡离子在根中的两个组织的节奏：根代谢物主要在黄昏或夜间达到高峰，而叶代谢物在白天达到高峰。许多振荡的代谢产物表现出组织特异性的调节，其中在未受攻击的组织中的系统反应特别明显的模拟草食动物。昼夜和草食引起的积累模式的二糖，苯丙氨酸，酪氨酸，石蒜苷I，香豆酰酪胺，12-oxophytodienoic酸和茉莉酸及其相关的生物合成成绩单进行了详细检查。我们的结论是，振荡代谢的N。envelopata积累在一个高度的组织特异性的方式和模式揭示了明显的昼夜节律的广义和专门的代谢介导的植物的反应草食动物和互利。我们建议，昼夜调节将被证明是一个重要的元素，在编排植物的反应，草食动物的攻击。
Ecological performance is all about timing and the endogenous clock that allows the entrainment of rhythms and anticipation of fitness-determining events is being rapidly characterized. How plants anticipate daily abiotic stresses, such as cold in early mornings and drought at noon, as well as biotic stresses, such as the timing of pathogen infections, is being explored, but little is known about the clock's role in regulating responses to insect herbivores and mutualists, whose behaviors are known to be strongly diurnally regulated and whose attack is known to reconfigure plant metabolomes. We developed a liquid chromatography-mass spectrometry procedure and analyzed its output with model-based peak picking algorithms to identify metabolites with diurnal accumulation patterns in sink/source leaves and roots in an unbiased manner. The response of metabolites with strong diurnal patterns to simulated attack from the specialist herbivore, Manduca sexta larvae was analyzed and annotated with in-house and public databases. Roots and leaves had largely different rhythms and only 10 ions of 182 oscillating ions in leaves and 179 oscillating ions in roots were rhythmic in both tissues: root metabolites mainly peaked at dusk or night, while leaf metabolites peaked during the day. Many oscillating metabolites showed tissue-specific regulation by simulated herbivory of which systemic responses in unattacked tissues were particularly pronounced. Diurnal and herbivory-elicited accumulation patterns of disaccharide, phenylalanine, tyrosine, lyciumoside I, coumaroyl tyramine, 12-oxophytodienoic acid and jasmonic acid and those of their related biosynthetic transcripts were examined in detail. We conclude that oscillating metabolites of N. attenuata accumulate in a highly tissue-specific manner and the patterns reveal pronounced diurnal rhythms in the generalized and specialized metabolism that mediates the plant's responses to herbivores and mutualists. We propose that diurnal regulation will prove to an important element in orchestrating a plant's responses to herbivore attack.