Isolation and characterization of a complementary DNA clone for a Mr 32,000 protein which is induced with tumor promoters in BALB/c 3T3 cells.
Isolation and characterization of a complementary DNA clone for a Mr 32,000 protein which is induced with tumor promoters in BALB/c 3T3 cells.
复制标题
在 BALB/c 3T3 细胞中用肿瘤启动子诱导的 Mr 32,000 蛋白的互补 DNA 克隆的分离和表征。
作者：
Hajime Kageyama;Takaki Hiwasa;K. Tokunaga;S. Sakiyama
The synthesis of a protein of Mr 32,000 (p32) is enhanced by various tumor promoters, chemical carcinogens, metal salts, and heat shock in BALB/c 3T3 cells. We have isolated a complementary DNA (cDNA) clone for p32 from a lambda gt10 library of BALB/c 3T3 cells. The library was constructed from mRNA extracted from the cells treated with sodium arsenite, which stimulates the p32 expression most effectively among various agents so far tested. Having screened this library differentially with probes which represent induced and uninduced mRNA populations for p32, we first obtained a partial p32 cDNA clone and have subsequently succeeded in the isolation of a cDNA clone containing the entire coding sequence. RNA blot analysis has shown that p32 mRNA is induced as early as 0.5 h after the addition of 12-O-tetradecanoyl-phorbol-13-acetate or sodium arsenite. Computer-assisted comparison with GenBank data has revealed a striking similarity in the nucleotide sequences between cDNAs of p32 and rat heme oxygenase. These results strongly suggest that p32 is a mouse homolog of this enzyme.
影响因子：
11.2
作者：
T. Hiwasa;H. Fujiki;T. Sugimura;S. Sakiyama
通讯作者：
T. Hiwasa;H. Fujiki;T. Sugimura;S. Sakiyama
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Shelton,KR;Todd,JM;Egle,PM
通讯作者：
Egle,PM
DOI：
10.1073/pnas.80.5.1194
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
YOUNG, RA;DAVIS, RW
通讯作者：
DAVIS, RW