Effect of Metal-Tolerant Plant Growth-Promoting Rhizobium on the Performance of Pea Grown in Metal-Amended Soil
Effect of Metal-Tolerant Plant Growth-Promoting Rhizobium on the Performance of Pea Grown in Metal-Amended Soil
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DOI：
10.1007/s00244-007-9097-y
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发表时间：
2008
影响因子：
4
通讯作者：
P. A. Wani;M. Khan;A. Zaidi
中科院分区：
文献类型：
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作者：
P. A. Wani;M. Khan;A. Zaidi
耐镍和锌的植物促生根瘤菌（PGP）。RP 5是从豌豆根瘤中分离出来的，豌豆生长在金属污染的印度土壤中。在体外条件下对菌株RP5的PGP活性进行了测定。菌株RP 5对镍（350 μg ml−1）和锌（1500 μg ml−1）表现出高水平的耐受性，并在体外条件下表现出PGP活性。使用豌豆作为测试作物，用增加浓度的镍和锌进一步评估该菌株的PGP活性。在290 mg Ni kg− 1土壤中，生物接种剂使干物质、根瘤数、根氮、芽氮、豆血红蛋白、种子产量和籽粒蛋白（GP）分别增加19%、23%、26%、47%、112%、26%和8%，而在4890 mg Zn kg− 1土壤中，生物接种剂增加了干物质、根瘤数、豆血红蛋白、种子产量、GP，根、地上部氮含量分别比对照提高18%、23%、78%、26%、7%、25%和42%。与未接种的植物相比，生物接种剂使根和根瘤的谷胱甘肽还原酶活性在580 mg Ni kg− 1土壤中分别增加了46%和65%，在9780 mg Zn kg− 1土壤中分别增加了47%和54%。接种菌株降低了植物器官中镍和锌的含量。供试菌株的固氮、促生长和降低镍、锌毒性的内在能力对提高豌豆在镍、锌污染土壤中的生长和产量具有重要的实际意义。
The nickel- and zinc-tolerant plant growth-promoting (PGP)Rhizobiumsp. RP5 was isolated from nodules of pea, grown in metal-contaminated Indian soils. The PGP potentials of strain RP5 was assessed underin vitroconditions. Strain RP5 displayed a high level of tolerance to nickel (350 μg ml−1) and zinc (1500 μg ml−1) and showed PGP activity underin vitroconditions. The PGP activity of this strain was further assessed with increasing concentrations of nickel and zinc, using pea as a test crop. The bio-inoculant enhanced the dry matter, nodule numbers, root N, shoot N, leghemoglobin, seed yield, and grain protein (GP) by 19%, 23%, 26%, 47%, 112%, 26%, and 8%, respectively, at 290 mg Ni kg−1while at 4890 mg Zn kg−1soil, it increased the dry matter, nodule numbers, leghemoglobin, seed yield, GP, and root and shoot N by 18%, 23%, 78%, 26%, 7%, 25%, and 42%, respectively, compared to plants grown in soil amended with metal only. The bio-inoculant increased the glutathione reductase activity of roots and nodules by 46% and 65% at 580 mg Ni kg−1and 47% and 54% at 9780 mg Zn kg−1soil, respectively, compared to uninoculated plants. The inoculated strain decreased the concentration of nickel and zinc in plant organs. The intrinsic abilities of nitrogen fixation, growth promotion, and the ability to reduce the toxicity of nickel and zinc of the tested strain could be of practical importance in augmenting the growth and yield of pea, in nickel- and zinc-polluted soils.