A modified electro-transformation method for Bacillus subtilis and its application in the production of antimicrobial lipopeptides
A modified electro-transformation method for Bacillus subtilis and its application in the production of antimicrobial lipopeptides
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枯草芽孢杆菌改良电转化方法及其在抗菌脂肽生产中的应用
DOI：
10.1007/s10529-011-0531-x
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发表时间：
2011-05-01
影响因子：
2.7
通讯作者：
Lu, Zhaoxin
中科院分区：
文献类型：
--
作者：
Cao, Guoqiang;Zhang, Xiaohui;Lu, Zhaoxin
提出了一种使用海藻糖的改良电穿孔方法用于枯草芽孢杆菌的转化。与常规方法相比，新方法将枯草芽孢杆菌的转化效率提高了近2000倍，得到4×10(5)个转化子/μg DNA。使用这种方法，枯草芽孢杆菌被改造以提高抗菌脂肽的产量，并产生 1.8 倍的表面活性素和 2.9 倍的风霉素。
A modified electroporation method using trehalose is presented for the transformation of Bacillus subtilis. The new method improved the transformation efficiency of B. subtilis nearly 2,000-fold compared with the usual method, giving 4 x 10(5) transformants/mu g DNA. Using this method, B. subtilis was engineered to improve production of antimicrobial lipopeptides and produced 1.8-fold more surfactin and 2.9-fold more fengycin.