Zonal uniformity in mechanical properties of the chondrocyte pericellular matrix: Micropipette aspiration of canine chondrons isolated by cartilage homogenization
Zonal uniformity in mechanical properties of the chondrocyte pericellular matrix: Micropipette aspiration of canine chondrons isolated by cartilage homogenization
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DOI：
10.1007/s10439-005-4479-7
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发表时间：
2005-10-01
影响因子：
3.8
通讯作者：
Setton, LA
中科院分区：
文献类型：
--
作者：
Guilak, F;Alexopoulos, LG;Setton, LA
细胞周基质（PCM）是关节软骨中包围软骨细胞的组织区域，与封闭的细胞一起称为软骨原。以前的研究表明，PCM的机械性能，相对于软骨细胞和细胞外基质（ECM），可能会显着影响应力应变，物理化学和流体流动环境的细胞。本研究的目的是测量机械隔离软骨的PCM的生物力学特性，并测试PCM的杨氏模量随关节软骨中的起源区（表面与中间/深）而变化的假设。软骨从犬膝关节软骨中提取，使用机械均质化，PCM的弹性特性进行了测定，使用微管抽吸结合软骨作为弹性不可压缩半空间，弹性可压缩双层，或弹性可压缩壳的理论模型。的PCM的杨氏模量显着高于分离的软骨细胞，但在一个数量级低于软骨ECM的报道。在表层软骨（24.0 +/- 8.9 kPa）和中/深层软骨（23.2 +/- 7.1 kPa）之间未观察到PCM的杨氏模量存在显著差异。结合以前的理论生物力学模型的软骨，这些研究结果表明，PCM显着影响关节软骨中的软骨细胞的力学环境，因此可能发挥作用，在调节细胞反应的微机械因素。
The pericellular matrix (PCM) is a region of tissue that surrounds chondrocytes in articular cartilage and together with the enclosed cells is termed the chondron. Previous studies suggest that the mechanical properties of the PCM, relative to those of the chondrocyte and the extracellular matrix (ECM), may significantly influence the stress-strain, physicochemical, and fluid-flow environments of the cell. The aim of this study was to measure the biomechanical properties of the PCM of mechanically isolated chondrons and to test the hypothesis that the Young's modulus of the PCM varies with zone of origin in articular cartilage (surface vs. middle/deep). Chondrons were extracted from articular cartilage of the canine knee using mechanical homogenization, and the elastic properties of the PCM were determined using micropipette aspiration in combination with theoretical models of the chondron as an elastic incompressible half-space, an elastic compressible bilayer, or an elastic compressible shell. The Young's modulus of the PCM was significantly higher than that reported for isolated chondrocytes but over an order of magnitude lower than that of the cartilage ECM. No significant differences were observed in the Young's modulus of the PCM between surface zone (24.0 +/- 8.9 kPa) and middle/deep zone cartilage (23.2 +/- 7.1 kPa). In combination with previous theoretical biomechanical models of the chondron, these findings suggest that the PCM significantly influences the mechanical environment of the chondrocyte in articular cartilage and therefore may play a role in modulating cellular responses to micromechanical factors.