Influence of Fimbriae on Bacterial Adhesion and Viscoelasticity and Correlations of the Two Properties with Biofilm Formation.
Influence of Fimbriae on Bacterial Adhesion and Viscoelasticity and Correlations of the Two Properties with Biofilm Formation.
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DOI：
10.1021/acs.langmuir.6b03764
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发表时间：
2017-01
期刊：
影响因子：
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通讯作者：
Huabin Wang;J. Wilksch;Ligang Chen;J. W. H. Tan;R. Strugnell;M. Gee
中科院分区：
文献类型：
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作者：
Huabin Wang;J. Wilksch;Ligang Chen;J. W. H. Tan;R. Strugnell;M. Gee
细菌的表面聚合物决定了细菌粘附到基质上进行定殖的能力，这是各种微生物过程（例如生物膜形成和生物污垢）的必要步骤。荚膜多糖和菌毛是细菌表面的两种主要成分，它们对于介导细胞-表面相互作用至关重要。细菌的粘附性和粘弹性是与细菌-表面相互作用相关的两个主要物理性质。在这项研究中，我们采用原子力显微镜（AFM）询问如何粘附工作和粘弹性的细菌病原体，肺炎克雷伯氏菌，影响生物膜的形成。为了达到这个目的，野生型、3型菌毛缺陷型和3型菌毛过表达的K。已经在水性环境中研究了肺炎杆菌菌株。结果表明，粘附功与生物膜的形成呈正相关，而粘弹性与生物膜的形成无关。该研究表明，基于AFM的细菌粘附测量可以用于评估细菌表面聚合物在生物膜形成中的功能，并预测细菌生物膜形成的能力。
The surface polymers of bacteria determine the ability of bacteria to adhere to a substrate for colonization, which is an essential step for a variety of microbial processes, such as biofilm formation and biofouling. Capsular polysaccharides and fimbriae are two major components on a bacterial surface, which are critical for mediating cell-surface interactions. Adhesion and viscoelasticity of bacteria are two major physical properties related to bacteria-surface interactions. In this study, we employed atomic force microscopy (AFM) to interrogate how the adhesion work and the viscoelasticity of a bacterial pathogen, Klebsiella pneumoniae, influence biofilm formation. To do this, the wild-type, type 3 fimbriae-deficient, and type 3 fimbriae-overexpressed K. pneumoniae strains have been investigated in an aqueous environment. The results show that the measured adhesion work is positively correlated to biofilm formation; however, the viscoelasticity is not correlated to biofilm formation. This study indicates that AFM-based adhesion measurements of bacteria can be used to evaluate the function of bacterial surface polymers in biofilm formation and to predict the ability of bacterial biofilm formation.