FAST-ATOM-BOMBARDMENT COMBINED WITH TANDEM MASS-SPECTROMETRY FOR THE STUDY OF DINUCLEOTIDES
FAST-ATOM-BOMBARDMENT COMBINED WITH TANDEM MASS-SPECTROMETRY FOR THE STUDY OF DINUCLEOTIDES
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DOI：
10.1016/0003-2697(86)90276-9
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发表时间：
1986-08-01
影响因子：
2.9
通讯作者：
GROTJAHN, L
中科院分区：
文献类型：
--
作者：
CERNY, RL;GROSS, ML;GROTJAHN, L
A study of mono- and dinucleotides by utilizing negative ion fast atom bombardment (FAB), metastable decomposition of (M-H)- species, and collisionally activated decomposition (CAD) of (M-H)- species is reported. Data were obtained for several complete series containing the standard nucleosides (guanosine, adenosine, cytidine, thymidine, and uridine): the 3''- and 5''-monophosphate mononucleotide series for both ribo- and 2''-deoxyribomononucleotides, all possible combinations for the 3''- .fwdarw. 5''-ribodinucleotides, and all possible combinations of the 3''- .fwdarw. 5'',2''-deoxyribodinucleotides. The metastable and CAD spectra provide more information than the FAB mass spectra. The (M-H)- ions of all dinucleotides decompose either as metastable ions or upon collisional activation to eliminate BH (B = base) preferentially from the 3''-rather than the 5''-terminus. Isomeric dinucleotides can be distinguished on the basis of this fragmentation. To establish the identity of the base at the 5''-terminus, collisional activation is preferred. By comparing relative abundances of BH elimination observed, the inherent basicities of the nucleoside base anions can be inferred to be C- > A-, T-, > G-.
