Thrombopoietin induces tyrosine phosphorylation and activation of mitogen-activated protein kinases in a human thrombopoietin-dependent cell line.
Thrombopoietin induces tyrosine phosphorylation and activation of mitogen-activated protein kinases in a human thrombopoietin-dependent cell line.
复制标题
血小板生成素在人血小板生成素依赖性细胞系中诱导酪氨酸磷酸化和丝裂原激活蛋白激酶的激活。
DOI：
10.1006/bbrc.1995.2768
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发表时间：
1995
影响因子：
3.1
通讯作者：
Yasusada Miura
中科院分区：
文献类型：
--
作者：
M. Yamada;Norio Komatsu;Koji Okada;T. Kato;Hiroshi Miyazaki;Yasusada Miura
血小板生成素（TPO）是一种细胞因子，可以支持巨核细胞祖细胞的增殖和分化，以及巨核细胞的成熟。我们在此表明丝裂原激活蛋白 (MAP) 激酶 Erk1 和 Erk2 参与人类 TPO 依赖性巨核细胞系 UT-7/TPO 中的 TPO 信号转导。 TPO 以剂量和时间依赖性方式诱导 Erk1 和 Erk2 蛋白酪氨酸磷酸化。此外，MAP激酶的激活实际上是由TPO诱导的。这些结果表明，MAP 激酶激活参与 TPO 的信号传导途径，就像其他细胞因子一样，其中之一是促红细胞生成素。
Thrombopoietin (TPO) is a cytokine which can support the proliferation and differentiation of megakaryocyte progenitor cells, and the maturation of megakaryocytes. We show here that mitogen-activated protein (MAP) kinases, Erk1 and Erk2, are involved in TPO signal transduction in the human TPO-dependent megakaryocytic cell line, UT-7/TPO. TPO induced tyrosine phosphorylation of Erk1 and Erk2 proteins in a dose and time-dependent manner. Moreover, the activation of MAP kinases was actually induced by TPO. These results suggest that MAP kinase activation is involved in the signalling pathway of TPO, as it is for other cytokines, one of which is erythropoietin.