MUTANT LAC REPRESSORS WITH NEW SPECIFICITIES HINT AT RULES FOR PROTEIN DNA RECOGNITION
MUTANT LAC REPRESSORS WITH NEW SPECIFICITIES HINT AT RULES FOR PROTEIN DNA RECOGNITION
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DOI：
10.1002/j.1460-2075.1990.tb08153.x
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发表时间：
1990-03-01
期刊：
影响因子：
11.4
通讯作者：
MULLERHILL, B
中科院分区：
文献类型：
--
作者：
LEHMING, N;SARTORIUS, J;MULLERHILL, B
识别特定 DNA 序列的蛋白质在所有生物体的蛋白质合成调节中发挥着重要作用。细菌蛋白 lac 阻遏物的特殊螺旋可识别 lac 操纵子大沟中的碱基。我们表明该识别螺旋的前两个残基独立地与两个碱基对相互作用。这使得我们在许多情况下能够预测抑制，作为抑制-操纵复合体强度的指标。可以推导出16个对称算子的识别规则。它们也适用于 gal 阻遏蛋白，并可能适用于其他细菌阻遏蛋白。
Proteins which recognize specific sequences of DNA play a fundamental role in the regulation of protein synthesis in all organisms. A particular helix of the bacterial protein lac repressor recognizes the bases in the major groove of the lac operator. We show that the first two residues of this recognition helix interact independently with two base pairs. This allows us in many cases to predict repression as an indicator of strength of the repressor-operator complex. Rules of recognition can be derived for 16 symmetric operators. They also apply to the gal repressor and possibly to other bacterial repressors.