Heterotrimeric G proteins regulate nitrogen-use efficiency in rice
Heterotrimeric G proteins regulate nitrogen-use efficiency in rice
复制标题
异三聚体 G 蛋白调节水稻的氮利用效率
DOI：
10.1038/ng.2958
复制
发表时间：
2014-06-01
期刊：
影响因子：
30.8
通讯作者：
Fu, Xiangdong
中科院分区：
文献类型：
--
作者：
Sun, Hongying;Qian, Qian;Fu, Xiangdong
迈向更可持续农业的努力提高了农作物养分利用效率的形象。在这里，我们表明，一个主要的水稻氮利用效率的数量性状位点（qNGR 9）是同义的先前确定的基因DEP 1（密集和直立穗1）。不同的DEP 1等位基因赋予不同的氮反应，遗传多样性分析表明，DEP 1在粳稻驯化过程中受到了人工选择。携带显性dep 1 - 1等位基因的植物表现出氮不敏感的营养生长，增加氮的吸收和同化，从而提高收获指数和粮食产量在中等水平的氮肥。DEP 1蛋白在体内与Gα（RGA 1）和Gβ（RGB 1）亚基相互作用，降低RGA 1或增强RGB 1活性抑制氮反应。我们的结论是，植物G蛋白复合物调节氮信号传导，异三聚体G蛋白活性的调节为环境可持续地增加水稻籽粒产量提供了一种策略。
The drive toward more sustainable agriculture has raised the profile of crop plant nutrient-use efficiency. Here we show that a major rice nitrogen-use efficiency quantitative trait locus (qNGR9) is synonymous with the previously identified geneDEP1(DENSE AND ERECT PANICLES 1). The differentDEP1alleles confer different nitrogen responses, and genetic diversity analysis suggests thatDEP1has been subjected to artificial selection duringOryza sativaspp.japonicarice domestication. The plants carrying the dominantdep1-1allele exhibit nitrogen-insensitive vegetative growth coupled with increased nitrogen uptake and assimilation, resulting in improved harvest index and grain yield at moderate levels of nitrogen fertilization. The DEP1 protein interactsin vivowith both the Gα (RGA1) and Gβ (RGB1) subunits, and reduced RGA1 or enhanced RGB1 activity inhibits nitrogen responses. We conclude that the plant G protein complex regulates nitrogen signaling and modulation of heterotrimeric G protein activity provides a strategy for environmentally sustainable increases in rice grain yield.