Mitochondrial membrane potential is dependent on the oligomeric state of F1F0-ATP synthase supracomplexes
Mitochondrial membrane potential is dependent on the oligomeric state of F1F0-ATP synthase supracomplexes
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DOI：
10.1074/jbc.m512334200
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发表时间：
2006-05-19
影响因子：
4.8
通讯作者：
Reichert, Andreas S.
中科院分区：
文献类型：
--
作者：
Bornhoevd, Carsten;Vogel, Frank;Reichert, Andreas S.
线粒体中的F1 F0-ATP合酶，除了其在能量转导中的功能外，在决定嵴形态中具有结构作用。这取决于其形成二聚体和更高的寡聚体超复合物的能力。在这里，我们表明，突变体的二聚体特异性亚基e和g，不稳定的二聚体和寡聚体F1 F0-ATP合酶超复合体，有一个降低的线粒体膜电位Δ Psi。不稳定的程度与膜电位的降低相关。F1 F0-ATP合酶和细胞色素c氧化酶的酶活性，最大呼吸速率，氧化磷酸化偶联，和管状线粒体形态没有受到影响或仅在较小程度上。在突变体缺乏一个或两个卷曲螺旋结构域的亚基e，线粒体膜电位的降低是不是由于线粒体DNA的损失，氧化磷酸化的能力降低，或改变嵴形态。我们提出了一个作用的超复合物的F1 F0-ATP合酶在组织内的内膜微区，确保最佳的生物能的能力的线粒体。
The F1F0-ATP synthase in mitochondria, in addition to its function in energy transduction, has a structural role in determining cristae morphology. This depends on its ability to form dimeric and higher oligomeric supracomplexes. Here we show that mutants of the dimer-specific subunits e and g, which destabilize dimeric and oligomeric F1F0-ATP synthase supracomplexes, have a decreased mitochondrial membrane potential Delta Psi. The degree of destabilization correlated with the reduction of the membrane potential. The enzymatic activities of F1F0-ATP synthase and cytochrome c oxidase, maximal respiration rate, coupling of oxidative phosphorylation, and tubular mitochondrial morphology were not affected or only to a minor extent. In mutants lacking one or two coiled-coil domains of subunit e, the reduction of the mitochondrial membrane potential was not due to loss of mitochondrial DNA, a reduced capacity of oxidative phosphorylation, or to altered cristae morphology. We propose a role for the supracomplexes of the F1F0-ATP synthase in organizing microdomains within the inner membrane, ensuring optimal bioenergetic competence of mitochondria.