Concentration-dependent behaviors of bone marrow derived mesenchymal stem cells and infectious bacteria toward magnesium oxide nanoparticles.
Concentration-dependent behaviors of bone marrow derived mesenchymal stem cells and infectious bacteria toward magnesium oxide nanoparticles.
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DOI：
10.1016/j.actbio.2016.02.032
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发表时间：
2016-04
影响因子：
9.7
通讯作者：
Cheyann Lee Wetteland;N. Nguyen;H. Liu
中科院分区：
文献类型：
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作者：
Cheyann Lee Wetteland;N. Nguyen;H. Liu
本文首次报道了氧化镁（MgO）纳米颗粒的浓度与其对哺乳动物细胞和感染性细菌的独特生物活性之间的定量关系。纳米氧化镁对骨髓间充质干细胞（BMSCs）和感染性细菌（革兰氏阴性大肠杆菌和革兰氏阳性表皮葡萄球菌）活力的影响在体外表现出浓度依赖性。本研究中确定的MgO纳米颗粒的临界浓度为生物材料设计提供了有价值的指导方针，以实现潜在的临床转化。与不含MgO的仅细胞对照相比，当在200 μg/mL MgO中培养时，BMSC密度显著增加。MgO浓度在500 μg/mL以上时，BMSCs密度明显降低。浓度等于或高于1000 μg/mL的MgO导致BMSC完全死亡。菌落形成单位（CFU）的定量显示MgO对E. coliandS.表皮生长因子1200 μg/mL。加入纳米MgO颗粒后，培养液中的pH值和Mg 2+离子浓度增加，这可能在观察到的细胞反应中起作用，但不是主要因素。coliandS.在碱性pH高达10或补充Mg 2+剂量高达50 mM时，表皮仍然显著增殖，表明MgO的杀菌特性超出了增加的培养基pH和Mg 2+离子浓度的影响。浓度为200 μg/mL的MgO纳米颗粒提供了促进BMSC增殖同时减少细菌粘附的双重益处，这对于潜在的医疗植入应用应进一步研究。游离MgO纳米颗粒的使用在高于300 μg/mL的浓度下对BMSC产生不利影响。本文首次报道了氧化镁（MgO）纳米颗粒浓度与其对哺乳动物细胞和感染性细菌的生物活性之间的定量关系。纳米氧化镁对骨髓间充质干细胞（BMSCs）和感染性细菌（革兰氏阴性大肠杆菌和革兰氏阳性表皮葡萄球菌）活力的影响在体外表现出浓度依赖性。本研究中确定的MgO纳米颗粒的临界浓度为生物材料设计提供了有价值的指导方针，以实现潜在的临床转化。
This article reports the quantitative relationship between the concentration of magnesium oxide (MgO) nanoparticles and its distinct biological activities towards mammalian cells and infectious bacteria for the first time. The effects of MgO nanoparticles on the viability of bone marrow derived mesenchymal stem cells (BMSCs) and infectious bacteria (both gram-negativeEscherichia coliand gram-positiveStaphylococcus epidermidis) showed a concentration-dependent behaviorin vitro. The critical concentrations of MgO nanoparticles identified in this study provided valuable guidelines for biomaterial design toward potential clinical translation. BMSCs density increased significantly when cultured in 200 μg/mL of MgO in comparison to the Cells Only control without MgO. The density of BMSCs decreased significantly after culture in the media with 500 μg/mL or more of MgO. Concentrations at or above 1000 μg/mL of MgO resulted in complete BMSCs death. Quantification of colony forming units (CFU) revealed that the minimum bactericidal concentration (MBC) of MgO forE. coliandS. epidermidiswas 1200 μg/mL. The addition of MgO nanoparticles into the cultures increased the pH and Mg2+ion concentration in the respective culture media, which might have played a role in the observed cell responses but not the main factors.E. coliandS. epidermidisstill proliferated significantly at alkaline pH up to 10 or with supplemental Mg2+dosages up to 50 mM, indicating bactericidal properties of MgO are beyond the effects of increased media pH and Mg2+ion concentrations. MgO nanoparticles at a concentration of 200 μg/mL provided dual benefits of promoting BMSC proliferation while reducing bacterial adhesion, which should be further studied for potential medical implant applications. The use of free MgO nanoparticles yielded detrimental effects to BMSCs in concentrations above 300 μg/mL. We recommend further study into MgO nanoparticle as a coating material or as a part of a composite.Statement of SignificanceThis article reports the quantitative relationship between the concentration of magnesium oxide (MgO) nanoparticles and its distinct biological activities towards mammalian cells and infectious bacteria for the first time. The effects of MgO nanoparticles on the viability of bone marrow derived mesenchymal stem cells (BMSCs) and infectious bacteria (both gram-negativeEscherichia coliand gram-positiveStaphylococcus epidermidis) showed a concentration-dependent behaviorin vitro. The critical concentrations of MgO nanoparticles identified in this study provided valuable guidelines for biomaterial design toward potential clinical translation.