Genetic analyses of the progeny of triploid gynogens induced from unreduced eggs of triploid (diploid female×tetraploid male) loach
Genetic analyses of the progeny of triploid gynogens induced from unreduced eggs of triploid (diploid female×tetraploid male) loach
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三倍体(二倍体雌性×四倍体雄性)泥鳅未减数卵诱导的三倍体雌性后代的遗传分析
DOI：
10.1016/s0044-8486(01)00843-2
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发表时间：
2002
期刊：
影响因子：
4.5
通讯作者：
K. Arai
中科院分区：
文献类型：
--
作者：
S. Momotani;K. Morishima;Quanqi Zhang;K. Arai
泥鳅中，三体（二倍体雌性×四倍体雄性）雌性既产下大尺寸的三体卵，又产下小尺寸的单倍体卵。用紫外线照射的精子激活大卵，获得第一代三倍体雌核发育细胞。4龄雌核母细胞均为雌性，可同时产1、2或3种配子：大型、小型和中型卵。第一代三倍体雌核发育个体之间的这种卵的频率不同。用紫外线照射的精子诱导大卵雌核发育，产生的第二代雌核细胞具有三倍体DNA含量。而由中等大小卵诱导的雌核发育细胞染色体数目和核型均为非整倍体（1.4-1.5n）。小型卵的雌核发育细胞在饲喂前死亡，可能是由于单倍体综合征的表达。用HinfI酶切后与寡核苷酸探针（GACA）4、（GGAT）4和33.15杂交，发现第二代雌核发育个体的DNA指纹图谱与母体完全一致，而另一些个体的DNA指纹图谱与母体略有不同。这些结果表明，在三倍体雌核发育过程中，既可形成无性系未减数卵，也可形成无性系未减数卵。
In the loach Misgurnus anguillicaudatus, triploid (diploid female×tetraploid male) females laid both large-sized triploid and small-sized haploid eggs. First-generation triploid gynogens were produced by activating the large-sized eggs with UV-irradiated spermatozoa. Mature 4-year-old gynogens were all females and laid one, two or three types of gametes, simultaneously: large-sized, small-sized and intermediate-sized eggs. Frequencies of such eggs differed among individuals of the first-generation triploid gynogens. When the large-sized eggs were triggered to induce gynogenetic development by UV-irradiated spermatozoa, the resultant second-generation gynogens had triploid DNA content. However, the gynogens induced from the intermediate-sized eggs exhibited aneuploid (1.4–1.5n) chromosome numbers and karyotypes. Gynogens from the small-sized eggs died before feeding, probably due to the expression of haploid syndrome. Among the second-generation triploid gynogens, some individuals revealed DNA fingerprints identical to the mother, but others gave patterns slightly different from the mother when the samples were digested with HinfI and then hybridized with oligonucleotide probes (GACA)4, (GGAT)4and 33.15. These results showed that both clonal and aclonal unreduced eggs might be formed in the triploid gynogens.