Expression patterns of four different regulatory genes that function during sea urchin development
Expression patterns of four different regulatory genes that function during sea urchin development
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DOI：
10.1016/j.modgep.2004.01.009
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发表时间：
2004-07-01
影响因子：
1.2
通讯作者：
Davidson, EH
中科院分区：
文献类型：
--
作者：
Minokawa, T;Rast, JP;Davidson, EH
编码四种不同转录因子的Strongylocentrotus purpuratus基因的空间和时间表达模式，即。 SpFoxb、SHes、SpKrl 和 SpNkl 已使用最近开发的高灵敏度整体原位杂交程序和定量实时 PCR 进行了检查。研究的两个基因 SpHes 和 SpNkl 是新分离出来的。它们的表达模式表明存在以前未知的外胚层结构域。对 SpFoxb 表达模式的重新检查揭示了该基因先前未报道的表达域，并且我们还提供了 SpKrl 表达模式比迄今为止更详细的时间和空间描述。 (C) 2004 Elsevier B.V. 保留所有权利。
The spatial and temporal expression patterns of Strongylocentrotus purpuratus genes encoding four different transcription factors, viz. SpFoxb, SpHes, SpKrl, and SpNkl, have been examined, using a recently developed, highly sensitive whole mount in situ hybridization procedure, and quantitative real time PCR. Two of the genes studied, SpHes and SpNkl, are newly isolated. Their expression patterns suggest the existence of previously unknown ectodermal domains. Re-examination of the expression pattern of SpFoxb reveals domains of expression not previously reported for this gene, and we also provide a more detailed, temporal and spatial description of the expression pattern of SpKrl than heretofore available. (C) 2004 Elsevier B.V. All rights reserved.