A cetyltrimethylammonium bromide-based method to extract low-molecular-weight RNA from polysaccharide-rich plant tissues
A cetyltrimethylammonium bromide-based method to extract low-molecular-weight RNA from polysaccharide-rich plant tissues
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基于十六烷基三甲基溴化铵的从富含多糖的植物组织中提取低分子量 RNA 的方法
DOI：
10.1016/j.ab.2006.09.022
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发表时间：
2007-01-15
影响因子：
2.9
通讯作者：
Schubert, Andrea
中科院分区：
文献类型：
--
作者：
Carra, Andrea;Gambino, Giorgio;Schubert, Andrea
综上所述，我们将CTAB法应用于从富含多糖的植物组织中提取LMW RNA。用该方法获得的LMW RNA纯度高、完整性好，可大量装载于变性PAGE中。因此，它可用于Northern杂交的siRNA检测和miRNA表达分析，并可用于从植物器官(如果实和种子)中产生小RNA文库，这些小RNA分子在其中的作用迄今鲜有人研究。
In summary, we have adapted the CTAB method to the extraction of LMW RNA from polysaccharide-rich plant tissues. LMW RNA obtained by the method presented is pure and intact, and can be loaded in large amount in denaturing PAGE. Thus, it can be used for siRNA detection and miRNA expression analysis by northern blotting, and to produce small RNA libraries from plant organs, such as fruits and seeds, in which the role of these small RNA molecules has been, thus far, little explored.