A SIMPLE IMMUNORADIOMETRIC ASSAY FOR THE TERMINAL SC5B-9 COMPLEX OF HUMAN-COMPLEMENT
A SIMPLE IMMUNORADIOMETRIC ASSAY FOR THE TERMINAL SC5B-9 COMPLEX OF HUMAN-COMPLEMENT
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DOI：
10.1016/0022-1759(83)90088-1
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发表时间：
1983-01-01
影响因子：
2.2
通讯作者：
MUHLY, M
中科院分区：
文献类型：
--
作者：
BHAKDI, S;MUHLY, M
通过经典或旁路途径激活血清中的补体，导致形成非共价结合的C5 b-C9补体成分和血清S蛋白的水溶性大分子SC 5 b-9蛋白复合物。特征性新抗原在该复合物上表达，其允许其与天然补体蛋白的免疫分化。本文描述了一种分离放射性标记的C5 b-9新抗原特异性抗体的方法。通过使用这些抗体，开发了一种简单而快速的SC 5 b-9免疫放射测定法，可以检测1 ml人血清或血浆中3-4 μ g的SC 5 b-9。该方法适用于患者血浆中SC 5 b-9的常规筛选分析，以及体外实验产生的SC 5 b-9的检测。
Complement activation in serum via classical or alternative pathway results in the formation of a water-soluble, macromolecular SC5b-9 protein complex of non-covalently associated C5b-C9 complement components and serum S-protein. Characteristic neoantigens are expressed on this complex that permit its immunological differentiation from native complement proteins. The present paper describes a procedure for isolating radiolabeled, specific antibodies to C5b-9 neoantigens. With the use of these antibodies, a simple and rapid immunoradiometric assay for SC5b-9 had been developed that allows for detection of 3-4 .mu.g SC5b-9 in 1 ml human serum or plasma. The method is suitable for routine screening analyses for SC5b-9 in patients'' plasma, and for detection of SC5b-9 experimentally generated in vitro.