Specialized functional domains in hemoglobin: dimensions in solution of the apohemoglobin dimer labeled with fluorescein iodoacetamide.
Specialized functional domains in hemoglobin: dimensions in solution of the apohemoglobin dimer labeled with fluorescein iodoacetamide.
复制标题
血红蛋白中的特殊功能域：用荧光素碘乙酰胺标记的脱辅基血红蛋白二聚体溶液中的尺寸。
DOI：
10.1021/bi00306a026
复制
发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
Steiner,RF
中科院分区：
文献类型：
--
作者：
Sassaroli,M;Bucci,E;Liesegang,J;Fronticelli,C;Steiner,RF
马西莫·萨萨罗利，**恩里科·布奇，*曾傑瑞·列塞冈，*克拉拉·弗兰蒂切利和罗伯特·F·施泰纳摘要：比较了8-苯基-1-萘磺酸(ANS)与脱脂血红蛋白和荧光素碘乙酰胺(FIA)标记脱脂血红蛋白(3-93)的荧光特性。用稳态和时间分辨技术测量了流动注射分析产生的ANS发射的猝灭。在这个体系中，ANS在/3-血红素口袋中的发射被FIA在(3-93)处完全熄灭。稳态测量表明，在血红素口袋中的ANS和FIA之间的能量转移效率为57%(8-93。时间分辨表明，ANS的初始(未猝灭)寿命为18.2 ns。在流动注射分析的存在下，产生了两个寿命分别为2.0 ns和6.6 ns的新组分。假设探针的随机取向，从这些测量中推断的距离对于时间分辨的接近4.6和3.6 nm，对于稳态测量接近48°。在血红蛋白的三维模型中，铁原子之间的距离
Massimo Sassaroli,** Enrico Bucci,* Jerry Liesegang,* Clara Fronticelli, and Robert F. Steiner abstract: The fluorescence characteristics of 8-anilino-naphthalene-1-sulfonic acid (ANS) coupled to apohemoglobin and to apohemoglobin labeled with fluorescein iodoacetamide (FIA) at (3-93 have been compared. The quenching of emission of ANS produced by FIA was measured both with steady-state and with time-resolved techniques. In thissystem the emission of ANS inthe/3-heme pockets was totally quenched by FIA at (3-93. Steady-state measurements indi-cated a 57% efficiency of energy transfer between ANS in the-heme pockets and FIA at (8-93. Time resolutionshowed that the initial (unquenched) lifetime of ANS was 18.2 ns. In the presence of FIA two new components were generated with lifetimes of 2.0 and 6.6 ns. Assuming a random orientation of the probes, the distances inferredfrom these measurements were near 4.6 and 3.6 nm for the time-resolved and near 48 Á for the steady-state measurements. In the tridimensional model of hemoglobin the distance between the iron atom of