Basophils Support the Survival of Plasma Cells in Mice
Basophils Support the Survival of Plasma Cells in Mice
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DOI：
10.4049/jimmunol.1002319
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发表时间：
2010-12-15
影响因子：
4.4
通讯作者：
Mack, Matthias
中科院分区：
文献类型：
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作者：
Gomez, Manuel Rodriguez;Talke, Yvonne;Mack, Matthias
我们先前已经表明，嗜碱性粒细胞通过增加B细胞增殖和IG产生以及诱导T细胞中的Th 2和B辅助表型来支持体液记忆免疫应答。基于脾和骨髓中嗜碱性粒细胞的高频率，在这项研究中，我们研究了嗜碱性粒细胞是否也支持浆细胞存活和IG产生。在缺乏嗜碱性粒细胞的情况下，初始或免疫小鼠的浆细胞在体外迅速发生细胞凋亡，仅产生少量的免疫球蛋白。相比之下，在嗜碱性粒细胞的存在下，甚至在活化的嗜碱性粒细胞的存在下，浆细胞的存活显著增加，并且能够持续产生Ig。这种作用部分依赖于嗜碱性粒细胞释放的IL-4和IL-6。当在存在或不存在激活嗜碱性粒细胞的物质的情况下培养总骨髓细胞或去除嗜碱性粒细胞的骨髓细胞时，获得了类似的结果。当嗜碱性粒细胞在体内耗尽后6个月的Ag免疫，特异性IG生产在随后的骨髓培养显着减少。此外，幼稚小鼠中嗜碱性粒细胞的耗竭18 d显著减少了脾脏中浆细胞的数量。这些数据表明，嗜碱性粒细胞是重要的浆细胞在体外和体内的生存。免疫学杂志，2010，185：7180-7185。
We have previously shown that basophils support humoral memory immune responses by increasing B cell proliferation and Ig production as well as inducing a Th2 and B helper phenotype in T cells. Based on the high frequency of basophils in spleen and bone marrow, in this study we investigated whether basophils also support plasma cell survival and Ig production. In the absence of basophils, plasma cells of naive or immunized mice rapidly undergo apoptosis in vitro and produce only low amounts of Igs. In contrast, in the presence of basophils and even more in the presence of activated basophils, the survival of plasma cells is markedly increased and continuous production of Igs enabled. This effect is partially dependent on IL-4 and IL-6 released from basophils. Similar results were obtained when total bone marrow cells or bone marrow cells depleted of basophils were cultured in the presence or absence of substances activating basophils. When basophils were depleted in vivo 6 mo after immunization with an Ag, specific Ig production in subsequent bone marrow cultures was significantly reduced. In addition, depletion of basophils for 18 d in naive mice significantly reduced the number of plasma cells in the spleen. These data indicate that basophils are important for survival of plasma cells in vitro and in vivo. The Journal of Immunology, 2010, 185: 7180-7185.