Transplantation of allogeneic or xenogeneic bone marrow within the donor stromal microenvironment
Transplantation of allogeneic or xenogeneic bone marrow within the donor stromal microenvironment
复制标题
DOI：
10.1097/00007890-199911150-00024
复制
发表时间：
1999-11-15
期刊：
影响因子：
6.2
通讯作者：
Slavin, S
中科院分区：
文献类型：
--
作者：
Gurevitch, O;Prigozhina, TB;Slavin, S
造血干细胞的成功植入需要支持性的造血基质微环境（HSM），与再生障碍性贫血、骨髓纤维化相关的HSM缺陷，或由强电离辐射和化疗引起的缺陷通常会导致骨髓（BM）植入失败。因此，在供体 HSM 内移植供体 BM 可能为同种异体 BM 提供最佳条件。移植。我们将供体造血细胞与其自身的 HSM 一起移植，以提高对同种异体或异种骨髓的接受度。非清髓治疗可诱导小鼠同种异体移植物的耐受性，并为终生接受同种异体 HSM 提供条件，肾包膜下自身 HSM 内移植的同种异体 BM 引起的移植物抗宿主病比静脉移植的 BM 少。小鼠对异种（大鼠）HSM 的耐受性不太完全。异位小骨较小且含有较少的造血细胞。然而，与单独移植BM相比，将大鼠BM和HSM同时移植到预处理的小鼠中改善了大鼠BM的植入。供体造血细胞在其自身的 HSM 上比在接受者的 HSM 上存活时间更长。
Successful engraftment of hematopoietic stem cells requires a supportive hematopoietic stromal microenvironment (HSM), Defects in the HSM associated with aplastic anemia, myelofibrosis, or caused by intensive ionizing radiation and chemotherapy generally result in failure of bone marrow (BM) engraftment. Transplantation of donor BM within donor HSM may therefore provide optimal conditions for allogeneic BM. transplantation. We have transplanted donor hematopoietic cells together with their own HSM to improve acceptance of allogeneic or xenogeneic BM. The non-myeloablative treatment used induced tolerance to murine allografts and provided conditions for the lifelong acceptance of allogeneic HSM, Allogeneic BM transplanted within it's own HSM under the kidney capsule caused less graft-versus-host disease than BM transplanted i.v. Tolerance in mice to xenogeneic (rat) HSM was less complete. Ectopic ossicles were small and contained fewer hematopoietic cells. However, simultaneous transplantation of rat BM and HSM to preconditioned mice improved engraftment of rat BM compared with transplantation of BM alone. Donor hematopoietic cells survived longer on their own HSM than on HSM of recipients.