Polylactic Acid Nanofiber Scaffold Decorated with Chitosan Islandlike Topography for Bone Tissue Engineering.
Polylactic Acid Nanofiber Scaffold Decorated with Chitosan Islandlike Topography for Bone Tissue Engineering.
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DOI：
10.1021/acsami.7b01176
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发表时间：
2017-06
影响因子：
9.5
通讯作者：
Ting Xu;Hongyan Yang;Dongzhi Yang;Zhongzhen Yu
中科院分区：
文献类型：
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作者：
Ting Xu;Hongyan Yang;Dongzhi Yang;Zhongzhen Yu
本研究采用静电纺丝技术，结合聚乳酸（PLA）和壳聚糖（CS）各自的优点，制备了一种具有核壳和岛状结构的双组分支架材料。本研究的目的是在聚乳酸静电纺丝纤维表面设计具有高生物活性的壳聚糖纳米形貌，以提高聚乳酸纤维膜的细胞生物相容性。对不同PLA/CS比例的纳米纤维进行了形态、内部结构、表面组成、结晶度和热力学分析，并推测了核壳或岛状形貌结构的转变机制。羟基磷灰石的矿化和前成骨细胞（MC 3 T3-E1）在改性支架上的培养结果表明，纳米纤维表面的CS组分和粗糙的纳米形貌平衡了纤维的亲水性和疏水性，增强了其矿化能力，更有利于细胞的附着和生长。此外，CS和“岛状”突起的纤维表面上增加了碱性磷酸酶的活性的MC 3 T3-E1细胞接种在纤维膜上，并提供了一个更合适的界面，细胞粘附和增殖。这些结果表明，这种聚乳酸/壳聚糖膜具有潜在的组织工程。更重要的是，我们的研究提供了一种新的方法来设计聚乳酸支架，在细胞和材料之间的界面结合的地形和生物活性修饰的效果，用于生物医学。
In this work, a bicomponent scaffold with a core-shell and islandlike structure that combines the respective advantages of polylactic acid (PLA) and chitosan (CS) was prepared via electrospinning accompanied by automatic phase separation and crystallization. The objective of this research was to design nanosized topography with highly bioactive CS onto PLA electrospun fiber surface to improve the cell biocompatibility of the PLA fibrous membrane. The morphology, inner structure, surface composition, crystallinity, and thermodynamic analyses of nanofibers with various PLA/CS ratios were carried out, and the turning mechanism of a core-shell or islandlike topography structure was also speculated. The mineralization of hydroxyapatite and culture results of preosteoblast (MC3T3-E1) cells on the modified scaffolds indicate that the outer CS component and rough nanoscale topography on the surface of the nanofibers balanced the hydrophilicity and hydrophobicity of the fibers, enhanced their mineralization ability, and made them more beneficial for the attachment and growth of cells. Moreover, CS and "islandlike" protrusions on the fiber surface increased the alkaline phosphatase activity of the MC3T3-E1 cells seeded on the fibrous membrane and provided a more appropriate interface for cell adhesion and proliferation. These results illustrate that this kind of PLA/CS membrane has the potential in tissue engineering. More importantly, our study provides a new approach to designing PLA scaffolds, with combined topographic and bioactive modification effects at the interface between cells and materials, for biomedicine.