Relationships between the expression of adipose genes and profiles of hospitalized dogs
Relationships between the expression of adipose genes and profiles of hospitalized dogs
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DOI：
10.1007/s11259-022-09989-2
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发表时间：
2022-09
影响因子：
2.2
通讯作者：
Yukina Sugiyama;Fumie Shimokawa;K. Sugiyama;Takashi Kobayashi;Yusuke Yamashita;Kei Kazama;K. Onda;M. Funaba;Masaru Murakami
中科院分区：
文献类型：
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作者：
Yukina Sugiyama;Fumie Shimokawa;K. Sugiyama;Takashi Kobayashi;Yusuke Yamashita;Kei Kazama;K. Onda;M. Funaba;Masaru Murakami
肥胖是犬发生多种代谢性疾病的危险因素之一。棕色/米色脂肪细胞中的能量消耗部分受骨形态发生蛋白（BMP）途径调节，是决定全身能量平衡的关键因素。在这里，我们研究了129只住院狗的脂肪库中的基因表达，以及基因表达的相对水平与狗的基因谱之间的关系。我们评估了23个基因的表达水平，如脂肪细胞分化和功能的调控基因，脂肪因子，棕色脂肪形成相关基因和解偶联蛋白（Ucp），以及参与BMP信号转导的基因。没有得到一个可靠的多元回归方程来解释身体状况评分（BCS），这是一个肥胖的指标。除Ucp 1和Ucp 3外，其他基因的表达水平均呈正相关。BCS随着年龄的增长而增加。BCS与Ppar γ、Fasn表达呈负相关，与Leptin、Opn 3表达呈正相关。衰老降低了脂肪细胞分化和功能相关基因（Pparγ、Fabp 4、Fasn、Hsl和Insr）和Adipoq的表达水平。此外，年龄与棕色脂肪形成和BMP信号成分（Prdm 16，Bmp 4，Alk 3，Actr 2a和Actr 2b）相关基因的表达呈负相关。Leptin和Ucp 2的表达水平随着年龄的增长而增加。本研究阐明了BCS和年龄相关的基因在脂肪组织中的表达，这可能有助于阐明犬肥胖的病因。
Obesity is one of the risk factors for the onset of various metabolic diseases in dogs. Energy expenditure in brown/beige adipocytes, which is partially regulated by the bone morphogenetic protein (BMP) pathway, is a key factor determining systemic energy balance. Here, we examined gene expression in the fat depots of 129 hospitalized dogs, and the relationship between the relative levels of gene expression and profiles of dogs. We evaluated the expression levels of 23 genes such as regulatory genes of adipocyte differentiation and function, adipokines, genes related to brown adipogenesis and uncoupling protein (Ucp), and genes involved in BMP signaling. A reliable equation of multiple regression was not obtained to explain the body condition score (BCS), which is an index of adiposity. Positive relationships were detected between the expression levels of many genes, except forUcp1orUcp3. BCS was found to increase with age. BCS was negatively correlated to the expression levels ofPparγandFasn, and positively correlated toLeptinandOpn3expression. Aging decreased the expression levels of genes related to adipocyte differentiation and function (Pparγ,Fabp4,Fasn,Hsl, andInsr) andAdipoq. In addition, age was negatively correlated with the expression of genes involved in brown adipogenesis and BMP signaling components (Prdm16,Bmp4,Alk3,Actr2a, andActr2b). In contrast, the expression levels ofLeptinandUcp2were found to increase with age. The present study clarifies BCS- and age-related gene expressions in the adipose tissue, which potentially contribute to elucidating the etiology of canine obesity.