Evaluation of different PCR assays for early detection of acute and relapsing brucellosis in humans in comparison with conventional methods
Evaluation of different PCR assays for early detection of acute and relapsing brucellosis in humans in comparison with conventional methods
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DOI：
10.1128/jcm.00010-06
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发表时间：
2007-04-01
影响因子：
9.4
通讯作者：
Kansouzidou, Athina
中科院分区：
文献类型：
--
作者：
Mitka, Stella;Anetakis, Constantine;Kansouzidou, Athina
人类布鲁氏菌病是包括希腊在内的许多地中海国家的一个重大公共卫生问题。传统的血清学方法，以及血培养，有严重的局限性，特别是在慢性，复发性和局灶性形式的疾病。对来自希腊塞萨洛尼基传染病医院的200名布鲁菌病患者的4，926份Huffy coat、全血和血清样品应用四种不同的PCR测定，用于快速诊断急性感染和复发，并与血液培养和血清学试验（即，Wright血清凝集试验、Coombs抗布鲁氏菌试验和补体结合试验）。四种PCR检测方法具有良好的敏感性和特异性，能够检测所有急性疾病病例。血沉棕黄层和全血是最佳标本。所有四种PCR检测在来自183名成功完成治疗的患者的所有随访样本中均为阴性，并且在来自17名在治疗后第一年复发的患者的所有随访样本中均为阳性，包括复发的次数。总之，PCR是一个非常有用的工具，用于快速诊断急性布鲁氏菌病和治疗后随访和早期发现复发的良好标志物。
Human brucellosis is a significant public health problem in many Mediterranean countries including Greece. The conventional serological methods, as well as blood cultures, have serious limitations, especially in chronic, relapsing, and focal forms of the disease. Four different PCR assays were applied in 4,926 huffy coat, whole-blood, and serum samples received from 200 patients admitted with brucellosis to the Infectious Diseases Hospital, Thessaloniki, Greece, for the rapid diagnosis of acute infection and relapses and compared to blood culture and serological tests (i.e., Wright's seroagglutination test, Coombs' antibrucella test, and the complement fixation test). The four PCR assays had excellent sensitivity and specificity and were able to detect all of the cases of acute disease. Buffy coat and whole blood were the optimal specimens. All four PCR assays were negative in all follow-up samples from 183 patients who had completed a successful treatment and were positive in all follow-up samples from 17 patients who had relapses in the first year after therapy, including the times of the relapses. In conclusion, PCR is a very useful tool for the rapid diagnosis of acute brucellosis and a good marker for the posttreatment follow-up and the early detection of relapses.