Unresponsive, a new behavioral mutant in Xenopus laevis
Unresponsive, a new behavioral mutant in Xenopus laevis
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无反应，非洲爪蟾的一种新行为突变体
DOI：
10.1111/j.1432-0436.1984.tb01394.x
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发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
R. Tompkins
中科院分区：
文献类型：
--
作者：
D. Reinschmidt;R. Tompkins
摘要 通过雌核发生和近交，从野生捕获的非洲爪蟾雌性中恢复了一种新的隐性非致命行为突变体无反应（ur）。突变胚胎直到三天大（即喂养开始前）才会移动，而正常胚胎则在尾芽阶段的一天开始移动。突变胚胎的恢复已完成但缓慢，还需要九天。移植分析表明，纯合突变胚胎中的感觉神经元功能正常，但来自体节的运动神经元及其目标肌肉都受到突变的影响。在与正常组织的嵌合体中，在完整突变胚胎恢复阶段之前，无反应胚胎的肌肉或运动神经元可以在早期发育过程中参与正常运动。突变体肌肉无法对乙酰胆碱做出正常反应，以及嵌合体的行为，表明突变体胚胎不会移动，因为它们在早期发育过程中不形成功能性神经肌肉接头，并且受这种突变影响的神经肌肉接头形成的组成过程通常是由神经和肌肉共同完成的。由于肌肉细胞缺陷而导致胚胎发生期间[5]。阿姆斯特朗和合作者 [I] 使用固定突变体来证明发育过程中胆碱能刺激引起的间隙连接丢失并不依赖于肌肉收缩。在这份报告中，我们研究了非洲爪蟾的一种新突变体，即无反应（ur），它直到进食阶段之前才会自主移动或响应刺激，之后就会恢复。
Abstract A new recessive nonlethal behavioral mutant, unresponsive (ur), was recovered from a wild-caught Xenopus laevis female by gynogenesis and inbreeding. Mutant embryos do not move until they are three days old, just before feeding begins, in contrast to normal embryos which begin movements at one day, during tailbud stage. Recovery of mutant embryos is complete but slow, requiring another nine days. Grafting analysis suggests that sensory neuron function is normal in homozygous mutant embryos, but that both motorneurons and their target muscles derived from somites are affected by the mutation. Either muscle or motorneurons of unresponsive embryos can participate in normal movements during early development, prior to the stage at which intact mutant embryos recover, in chimerae with normal tissue. Failure of mutant muscle to respond normally to acetylcholine, along with the behavior of chimerae, suggests that mutant embryos do not move because they do not form functional neuromuscular junctions during early development and that the component process of neuromuscular junction formation affected by this mutation is normally performed by both nerve and muscle. during embryogenesis due to a defect in the muscle cells [5]. Armstrong and collaborators [I] have used the immobile mutant to demonstrate that the cholinergic stimulation- induced loss of gap junctions during development does not depend on muscle contraction. In this report, we examine a new mutant of Xenopus laevis, unresponsive (ur), which does not move voluntarily or in response to stimulation until just prior to the feeding stage, after which it recovers.
培养物中受神经支配和非神经支配的非洲爪蟾肌肉细胞的乙酰胆碱敏感性。
影响因子：
2.7
作者：
Gruener,R;Kidokoro,Y
通讯作者：
Kidokoro,Y
培养物中神经支配和非神经支配的非洲爪蟾肌肉细胞上α-银环蛇毒素结合位点的分布和密度。
影响因子：
2.7
作者：
Kidokoro,Y;Gruener,R
通讯作者：
Gruener,R