Effects of methylprednisolone on glomerular and medullary mRNA levels for extracellular matrices in puromycin aminonucleoside nephrosis.
Effects of methylprednisolone on glomerular and medullary mRNA levels for extracellular matrices in puromycin aminonucleoside nephrosis.
复制标题
甲基泼尼松龙对嘌呤霉素氨基核苷肾病肾小球和髓质细胞外基质 mRNA 水平的影响。
DOI：
10.1038/ki.1991.288
复制
发表时间：
1991
影响因子：
19.6
通讯作者：
H. Koide
中科院分区：
文献类型：
--
作者：
T. Nakamura;I. Ebihara;M. Fukui;Y. Tomino;H. Koide
我们研究了甲基强的松龙（MPSL）对嘌呤霉素氨基糖苷（PAN）肾病的肾小球和髓质中IV型胶原、层粘连蛋白和硫酸乙酰肝素蛋白聚糖（HSPG）mRNA水平的影响。PAN肾病大鼠肾小球IV型胶原和层粘连蛋白mRNA表达明显增加，而HSPG mRNA表达明显下降。MPSL的管理部分改善异常基因表达的基底膜成分。此外，我们发现，所有这些基底膜成分的髓质mRNA水平随着年龄的增加而下降，在PAN肾病与或没有MPSL治疗，这表明无论是PAN或MPSL对基底膜成分的mRNA水平在肾髓质有任何影响。与此相反，mRNA水平的间质胶原蛋白，包括α 1（I）和α 1（III）链在肾小球中表现出很小的变化与或没有MPSL治疗，而那些在髓质增加显着PAN肾病与对照组相比。MPSL可改善肾髓质α 1（I）和α 1（III）胶原基因的异常表达。这些结果表明，PAN影响肾小球mRNA编码的基底膜成分和髓质mRNA编码的间质胶原，MPSL有显着的改善PAN肾病肾小球和髓质的异常基因表达。
We examined the effects of methylprednisolone (MPSL) on type IV collagen, laminin and heparan sulfate proteoglycan (HSPG) mRNA levels in the renal glomeruli and medulla of puromycin aminonucleoside (PAN) nephrosis. mRNA levels encoding for type IV collagen and laminin increased markedly, whereas those for HSPG decreased significantly in glomeruli of PAN nephrosis. Administration of MPSL partially ameliorated the abnormal gene expression for basement membrane components. Furthermore, we showed that medullary mRNA levels for all these basement membrane components decreased with age in PAN nephrosis with or without MPSL treatment, suggesting that neither PAN nor MPSL has any effect on basement membrane component mRNA levels in the renal medulla. In contrast, mRNA levels for the interstitial collagens including alpha 1 (I) and alpha 1 (III) chains in glomeruli showed little change with or without MPSL treatment, whereas those in medulla increased significantly in PAN nephrosis when compared with the control. MPSL ameliorated the abnormal gene expression of alpha 1 (I) and alpha 1 (III) collagen in renal medulla. These results indicate that PAN affects both glomerular mRNA encoding for basement membrane components and medullary mRNA encoding for interstitial collagens, and that MPSL has marked effects on the amelioration of abnormal gene expression in both glomeruli and medulla of PAN nephrosis.