The superimposed effects of chronic phrenicotomy and cervical spinal cord hemisection on synaptic cytoarchitecture in the rat phrenic nucleus
The superimposed effects of chronic phrenicotomy and cervical spinal cord hemisection on synaptic cytoarchitecture in the rat phrenic nucleus
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DOI：
10.1006/exnr.1997.6452
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发表时间：
1997-05-01
影响因子：
5.3
通讯作者：
Goshgarian, HG
中科院分区：
文献类型：
--
作者：
Liou, WW;Goshgarian, HG
本研究的目的是确定联合外周膈切开术和头端脊髓半切术对同侧大鼠膈核突触结构的影响。年轻成年雌性 Sprague-Dawley 大鼠被分为单独半切组和两个半切加膈肌切开术 (HPP) 组。在所有动物中，将 DiI（一种荧光碳花青染料）注射到左侧膈肌中，以逆行标记同侧膈运动神经元。在HPP组中，在处死前2周和4周进行左胸内膈切开术，仅半切动物不进行膈切开术。在所有动物中，死亡前 24 小时进行左 C2 脊髓半切。膈核的定量形态测量分析表明，与单独半切断组相比，HPP（2周）组中接触膈轮廓的突触数量显着减少，但该数量恢复到与HPP（4周）组中单独半切断值没有显着差异的水平。结果表明，突触数量的瞬时变化可能导致另一组接受相同外科手术的动物中记录的交叉膈现象的差异表达。此外，膈切开术/脊髓半切诱导的神经胶质反应的不同阶段可能是突触数量变化的基础。 (C) 1997 年学术出版社。
The present study was carried out to determine the effects of a combined peripheral phrenicotomy and rostral spinal cord hemisection on the synaptic architecture in the ipsilateraI rat phrenic nucleas. Young adult female Sprague-Dawley rats were divided into a hemisection-alone and two hemisection-plus-phrenicotomy (HPP) groups. In all animals, DiI, a fluorescent carbocyanine dye was injected into the left hemidiaphragm to retrogradely label the ipsilateral phrenic motoneurons. ill the HPP groups, left intrathoracic phrenicotomies were carried out at 2 and 4 weeks prior to sacrificing, Hemisection-alone animals were not subjected to phrenicotomy. In all animals, a left C2 spinal cord hemisection was performed 24 h prior to death. Quantitative morphometric analysis of the phrenic nucleus showed that the number of synapses contacting phrenic profiles is significantly less in the HPP (2 week) group as compared to the hemisection-alone group, but this number returns to a level not significantly different from the hemisection-alone value in the HPP (4 week) group. The results suggest that the transient change in the number of synapses might contribute 60 the differential expression of the crossed phrenic phenomenon documented in another group of animals subjected 60 the same surgical procedures. Furthermore, the different stages of glial reaction induced by phrenicotomy/spinal cord hemisection might underlie the change in synaptic number. (C) 1997 Academic Press.